Anhui Agricultural University 3 articles published in JoVE Chemistry Tea Aroma Analysis Based on Solvent-Assisted Flavor Evaporation Enrichment Zhihui Feng1, Xiaogen Yang2, Chun Zou1, Junfeng Yin1 1Tea Research Institute Chinese Academy of Agricultural Sciences, 2State Key Laboratory of Tea Plant Biology and Utilization, Anhui Agricultural University Presented here is a method for enriching and analyzing the volatile components of tea extracts using solvent-assisted flavor evaporation and solvent extraction followed by gas chromatography-mass spectrometry, which can be applied to all types of tea samples. Environment Source and Route of Pyrrolizidine Alkaloid Contamination in Tea Samples Weiting Jiao1,2, Tingting Shen2, Luyao Wang1, Lei Zhu1,3, Qing X. Li4, Chen Wang3, Hongping Chen3, Rimao Hua1, Xiangwei Wu1 1Key Laboratory of Agri-food Safety of Anhui Province, School of Resource & Environment, Anhui Agricultural University, 2State Key Laboratory of Tea Plant Biology and Utilization, School of Tea and Food Science & Technology, Anhui Agricultural University, 3Key Laboratory of Tea Quality and Safety & Risk Assessment, Tea Research Institute, Chinese Academy of Agricultural Sciences, Ministry of Agriculture, 4Department of Molecular Biosciences and Bioengineering, University of Hawaii at Manoa The present protocol describes the contamination of pyrrolizidine alkaloids (PAs) in tea samples from PA-producing weeds in tea gardens. Biology Agrobacterium-Mediated Genetic Transformation, Transgenic Production, and Its Application for the Study of Male Reproductive Development in Rice Dawei Xu*1,2, Palash Chandra Mondol*1, Muhammad Uzair1, Matthew R. Tucker3, Dabing Zhang1,3 1Joint International Research Laboratory of Metabolic and Developmental Sciences, Shanghai Jiao Tong University-University of Adelaide Joint Centre for Agriculture and Health, School of Life Sciences and Biotechnology, Shanghai Jiao Tong University, 2College of Agronomy, Anhui Agricultural University, Heifei, China, 3School of Agriculture, Food and Wine, University of Adelaide This work describes the use of CRISPR-Cas9 genome editing technology to knockout endogenous gene OsABCG15 followed by a modified Agrobacterium-mediated transformation protocol to produce a stable male-sterile line in rice.
