Guangdong Laboratory Animals Monitoring Institute 3 articles published in JoVE Genetics Investigating the Pathogenesis of MYH7 Mutation Gly823Glu in Familial Hypertrophic Cardiomyopathy using a Mouse Model Yu Xia*1,2, Jinlin Hu*3, Xiang Li4, Shuang Zheng4, Ge Wang1,2, Songtao Tan1,2, Zengxiao Zou1,2, Qiong Ling2,5, Fenghua Yang4, Xiaoping Fan1,2 1Department of Cardiovascular Surgery, Guangdong Provincial Hospital of Traditional Chinese Medicine, the Second Affiliated Hospital of Guangzhou University of Chinese Medicine, 2The Second Clinical College of Guangzhou University of Chinese Medicine, 3Department of Cardiovascular Surgery, The First Affiliated Hospital, Jinan University, 4Guangdong Provincial Key Laboratory of Laboratory Animals, Guangdong Laboratory Animals Monitoring Institute, 5Department of Anesthesiology, Guangdong Provincial Hospital of Chinese Medicine, the Second Affiliated Hospital of Guangzhou University of Chinese Medicine Based on the familial hereditary cardiomyopathy family found in our clinical work, we created a C57BL/6N mouse model with a point mutation (G823E) at the mouse MYH7 locus through CRISPR/Cas9-mediated genome engineering to verify this mutation. Medicine Establishment and Evaluation of a Porcine Vein Graft Disease Model Xiaohui Li*1, Jinlin Hu*1, Weijiang Tan*2, Zhaoming Lin*1, Caiyi Zhu2, Chengfeng Huang1, Jiawen Huang3, Yintian Liu1, Qiuying Liao1, Hua Lu1, Xiaoshen Zhang1 1Department of Cardiovascular Surgery, the First Affiliated Hospital of Jinan University, 2Guangdong Provincial Key Laboratory of Laboratory Animals, Guangdong Laboratory Animals Monitoring Institute, 3The First Affiliated Hospital of Jinan University In this protocol, novel pig vein bypass grafting was performed through a small incision in the left chest wall without cardiopulmonary bypass. A postoperative pathology study was done, which showed intimal thickening. Medicine A Surgical Model of Heart Failure with Preserved Ejection Fraction in Tibetan Minipigs Xiaohui Li*1,2, Weijiang Tan*1,3, Xiang Li*1, Shuang Zheng*1, Xiaoshen Zhang2, Honghua Chen1, Zhanhong Pan1, Caiyi Zhu1, Feng Hua Yang1 1Guangdong Laboratory Animals Monitoring Institute, 2Department of Cardiovascular Surgery, the First Affiliated Hospital, Jinan University, 3College of Veterinary Medicine, South China Agricultural University The present protocol describes a step-by-step procedure to establish a minipig model of heart failure with preserved ejection fraction using descending aortic constriction. The methods for evaluating cardiac morphology, histology, and function of this disease model are also presented.
