Osaka City University Graduate School of Medicine 5 articles published in JoVE Biology Isolation of Retinal Pigment Epithelial Cells from Guinea Pig Eyes So Goto1,2,3, Michael Frost4, Christine Wildsoet1 1Herbert Wertheim School Optometry and Vision Science, University of California, Berkeley, 2Department of Ophthalmology, Osaka University Graduate School of Medicine, 3Department of Ophthalmology, Tokyo Medical Center, National Hospital Organization, 4Department of Optometry and Vision Science, School of Optometry, University of Alabama at Birmingham We describe a simple and efficient method for isolating cells of the retinal pigment epithelium (RPE) cells from the eyes of young pigmented guinea pigs. This procedure allows for follow-up molecular biology studies on the isolated RPE, including gene expression analyses. Immunology and Infection Bone Marrow Transplantation Procedures in Mice to Study Clonal Hematopoiesis Eunbee Park1, Megan A. Evans4, Heather Doviak4, Keita Horitani4, Hayato Ogawa4, Yoshimitsu Yura4, Ying Wang2, Soichi Sano3,4, Kenneth Walsh1,4 1Department of Biochemistry and Molecular Genetics, University of Virginia School of Medicine, 2Department of Cardiology, Xinqiao Hospital, Army Medical University, 3Department of Cardiology, Osaka City University Graduate School of Medicine, 4Hematovascular Biology Center, Robert M. Berne Cardiovascular Research Center, University of Virginia School of Medicine We describe three methods of bone marrow transplantation (BMT): BMT with total-body irradiation, BMT with shielded irradiation, and BMT method with no pre-conditioning (adoptive BMT) for the study of clonal hematopoiesis in mouse models. Neuroscience Reliable Acquisition of Electroencephalography Data during Simultaneous Electroencephalography and Functional MRI Hui Ming Khoo1, Yuya Fujita1, Naoki Tani1, Tetsuya Shimokawa2, Natalja Zazubovits3, Satoru Oshino1, Jean Gotman3, Haruhiko Kishima1 1Department of Neurosurgery, Osaka University Graduate School of Medicine, 2Center for Information and Neural Networks, National Institute of Information and Communications Technology, 3Montreal Neurological Institute and Hospital, McGill University This article provides a straightforward protocol for acquiring good quality electroencephalography (EEG) data during simultaneous EEG and functional magnetic resonance imaging by utilizing readily available medical products. Medicine Monitoring PD-1-Blocking Antibodies Bound to T Cells Derived from a Drop of Peripheral Blood Yujiro Naito1, Akio Osa1, Kentaro Masuhiro1, Takashi Hirai1, Shohei Koyama1, Atsushi Kumanogoh1 1Department of Respiratory Medicine and Clinical Immunology, Osaka University Graduate School of Medicine We developed a simple flow cytometry assay for evaluating the binding of PD-1–blocking antibodies to T cells, requiring only a drop of peripheral blood from cancer patients. Cancer Research Generation of a Liver Orthotopic Human Uveal Melanoma Xenograft Platform in Immunodeficient Mice Ken Kageyama1,3, Shinji Ozaki2,3, Takami Sato3 1Department of Diagnostic and Interventional Radiology, Osaka City University Graduate School of Medicine, 2Department of Surgery, National Hospital Organization, Kure Medical Cancer Center, 3Department of Medical Oncology, Sidney Kimmel Cancer Center, Thomas Jefferson University Orthotopic human liver metastatic uveal melanoma xenograft mouse models were created using surgical orthotopic implantation techniques with patient-derived tumor chunk and needle injection techniques with cultured human uveal melanoma cell lines.
