KAIST 6 articles published in JoVE Neuroscience In Vivo Whole-Brain Imaging of Zebrafish Larvae Using Three-Dimensional Fluorescence Microscopy Eun-Seo Cho1, Seungjae Han1, Gyuri Kim1, Minho Eom1, Kang-Han Lee2, Cheol-Hee Kim2, Young-Gyu Yoon1,3 1School of Electrical Engineering, KAIST, 2Department of Biology, Chungnam National University, 3KAIST Institute for Health Science and Technology Presented here is a protocol for in vivo whole-brain imaging of larval zebrafish using three-dimensional fluorescence microscopy. The experimental procedure includes sample preparation, image acquisition, and visualization. Neuroscience A Novel In Vitro Live-imaging Assay of Astrocyte-mediated Phagocytosis Using pH Indicator-conjugated Synaptosomes Youkyeong Gloria Byun1, Won-Suk Chung1 1Department of Biological Sciences, Korea Advanced Institute of Science and Technology This protocol presents an in vitro live-imaging phagocytosis assay to measure the phagocytic capacity of astrocytes. Purified rat astrocytes and microglia are used along with pH indicator-conjugated synaptosomes. This method can detect real-time engulfment and degradation kinetics and provides a suitable screening platform to identify factors modulating astrocyte phagocytosis. Neuroscience Implantation of Chronic Silicon Probes and Recording of Hippocampal Place Cells in an Enriched Treadmill Apparatus Anvar Sariev1,2, Jinho Chung1, Dajung Jung1,3, Farnaz Sharif1,2, Ju-Young Lee1,2, Soyoun Kim1, Sebastien Royer1,2 1Center for Functional Connectomics, Korea Institute of Science and Technology, 2Biomedical department, KIST school, University of Science and Technology, 3Department of Biological Sciences, Korea Advanced Institute of Science and Technology We describe the diverse steps to implant chronic silicon probes and to record place cells in mice that are running head-fixed on a cue-enriched treadmill apparatus. Engineering Experimental Implementation of a New Composite Fabrication Method: Exposing Bare Fibers on the Composite Surface by the Soft Layer Method Dongyoung Lee1, Dai Gil Lee1, Jun Woo Lim2 1Department of Mechanical Engineering, Korea Advanced Institute of Science and Technology, 2LANL-CBNU Engineering Institute Korea, Chonbuk National University A protocol to expose bare fibers on the composite surface by eliminating resin rich area is presented. The fibers are exposed during fabrication of the composites, not by the post surface treatment. The exposed carbon composites exhibit high electrical conductivity in the through-thickness direction and high mechanical property. Bioengineering TAPE: A Biodegradable Hemostatic Glue Inspired by a Ubiquitous Compound in Plants for Surgical Application Keumyeon Kim1, Haeshin Lee1,2, Seonki Hong2,3 1The Graduate School of Nanoscience and Technology, Korea Advanced Institute of Science and Technology (KAIST), 2Department of Chemistry, Korea Advanced Institute of Science and Technology (KAIST), 3Center for Systems Biology, Massachusetts General Hospital/Harvard Medical School We describe the simplest protocol to prepare biodegradable medical glue that has an effective hemostatic ability. TAPE is a water-immiscible supramolecular aggregate prepared by mixing of tannic acid, a ubiquitous compound found in plants, and poly(ethylene) glycol, yielding a 2.5 times greater water-resistant adhesion compared with commercial fibrin glue. Bioengineering Fluorescence Recovery after Merging a Droplet to Measure the Two-dimensional Diffusion of a Phospholipid Monolayer Dae-Woong Jeong1, KyuHan Kim2, Myung Chul Choi1, Siyoung Q. Choi2 1Department of Bio and Brain Engineering, KAIST, 2Information and Electrical Research Institute, KAIST We present a new technique to measure the lateral diffusion of a surface active species at the fluid-fluid interface by merging a droplet monolayer onto a flat monolayer.
