Universite Catholique de Louvain 3 articles published in JoVE Biology The Chick Chorioallantoic Membrane (CAM) Model as a Tool to Study Ovarian Tissue Transplantation Camille Hossay1, Luciana Cacciottola1, Sarah Storder1, Olivier Van Kerk1, Marie-Madeleine Dolmans1,2 1Gynecology Research Unit, Institut de Recherche Expérimentale et Clinique, Université Catholique de Louvain, 2Gynecology Department, Cliniques Universitaires Saint-Luc Here, we describe a protocol for developing a chick chorioallantoic membrane (CAM) xenografting model for human ovarian tissue and demonstrate the effectiveness of the technique, the graft revascularization time frame, and the tissue viability across a 6 day grafting period. Cancer Research Multiplex Immunofluorescence Combined with Spatial Image Analysis for the Clinical and Biological Assessment of the Tumor Microenvironment Nicolas Huyghe1, Elena Benidovskaya1, Simon Beyaert1, Aurélie Daumerie4, Finoula Maestre Osorio1, Frank Aboubakar Nana2,3, Caroline Bouzin4, Marc Van den Eynde1,5 1Institut de Recherche Expérimentale et Clinique (IREC), Pôle MIRO, Université Catholique de Louvain (UCLouvain), 2Institut de Recherche Expérimentale et Clinique (IREC), Pôle de Pneumologie, ORL et Dermatologie (PNEU), Université Catholique de Louvain (UCLouvain), 3Division of Pneumology, Cliniques Universitaires St-Luc, Université Catholique de Louvain (UCLouvain), 4IREC Imaging Platform, Université Catholique de Louvain (UCLouvain), 5Institut Roi Albert II, Department of Medical Oncology and Gastroenterology, Cliniques Universitaires St-Luc In this article, a protocol for manual tyramide signal amplification (TSA) multiplex immunofluorescence (mIF) combined with image analysis and spatial analysis is described. This protocol can be used with formalin-fixed paraffin-embedded (FFPE) sections for the staining of two to six antigens per slide depending on the slide scanner available in the laboratory. Medicine Fiber Type and Subcellular-Specific Analysis of Lipid Droplet Content in Skeletal Muscle Camille M. Selvais1, Laura L. De Cock1, Sonia M. Brichard1, María A. Davis-López de Carrizosa1,2 1Endocrinology, Diabetes and Nutrition Unit, Institute of Experimental and Clinical Research, Medical Sector, Université Catholique de Louvain, 2Departamento de Fisiología, Facultad de Biología, Universidad de Sevilla Increasing evidence indicates that excessive infiltration of lipids inside skeletal muscle results in lipotoxicity and diabetes. Here, we present a complete protocol, including tissue processing, staining with Bodipy, image acquisition, and analysis, to quantify the size, density, and subcellular distribution of lipid droplets in a fiber-type specific manner.
