University of Zaragoza 3 articles published in JoVE Cancer Research Transmitochondrial Cybrid Generation Using Cancer Cell Lines Ruth Soler-Agesta1, Joaquín Marco-Brualla1, Patricio Fernández-Silva1,2, Pilar Mozas1,3, Alberto Anel1, Raquel Moreno Loshuertos1,2 1Department of Biochemistry, University of Zaragoza, 2Institute for Biocomputation and Physics of Complex Systems, University of Zaragoza, 3Sequencing and Functional Genomics Unit, University of Zaragoza-IACS This protocol describes a technique for cybrid generation from suspension-growing cancer cells as a tool to study the role of mitochondria in the tumorigenic process. Biochemistry Improved UPLC-UV Method for the Quantification of Vitamin C in Lettuce Varieties (Lactuca sativa L.) and Crop Wild Relatives (Lactuca spp.) Inés Medina-Lozano*1,4, Juan Ramón Bertolín*2,4, Raquel Zufiaurre3, Aurora Díaz1,4 1Unidad de Hortofruticultura, Centro de Investigación y Tecnología Agroalimentaria de Aragón (CITA), 2Unidad de Producción y Sanidad Animal, Centro de Investigación y Tecnología Agroalimentaria de Aragón (CITA), 3Dpto Química Analítica, Escuela Politécnica Superior (Universidad de Zaragoza), 4Instituto Agroalimentario de Aragón - IA2 (CITA-Universidad de Zaragoza) We present a fast and reliable method to quantify vitamin C in Lactuca spp. using UPLC-UV, potentially transferable to other plants. The key steps are the sample preparation and vitamin C extraction under stable conditions, the reduction of dehydroascorbic acid to ascorbic acid and the optimization of the chromatographic procedure. Immunology and Infection Protective Efficacy and Pulmonary Immune Response Following Subcutaneous and Intranasal BCG Administration in Mice Santiago Uranga1, Dessislava Marinova1, Carlos Martin1, Nacho Aguilo1 1Department of Microbiology, University of Zaragoza We herein detail the methodology followed to compare protective efficacy and lung immune response induced by intranasal and subcutaneous immunization with BCG in mouse model. Our results show the benefits of pulmonary vaccination and suggest a role for IL17-mediated response in vaccine-induced protection.
