Consejo Superior de Investigaciones Cientificas 4 articles published in JoVE Environment Automatic Image Processing to Determine the Community Size Structure of Riverine Macroinvertebrates Rosa Gurí1,4, Ignasi Arranz2,4, Marc Ordeix1,4, Carmen García-Comas3,4 1Center for the Study of Mediterranean Rivers (CERM), Universitat de Vic - Universitat Central de Catalunya, 2Laboratoire Evolution et Diversité Biologique (EDB), UMR5174, Université Toulouse 3 Paul Sabatier, Centre national de la recherche scientifique (CNRS), Institut de Recherche pour le Développement (IRD), 3Department of Marine Biology and Oceanography, Institut de Ciències del Mar, Consejo Superior de Investigaciones Científicas (CSIC), 4Aquatic Ecology Group, Universitat de Vic - Universitat Central de Catalunya The article is based on the creation of an adapted protocol to scan, detect, sort, and identify digitized objects corresponding to benthic river macroinvertebrates using a semi-automatic imaging procedure. This procedure allows the acquisition of the individual size distributions and size metrics of a macroinvertebrate community in about 1 h. Cancer Research Fibroblast-Derived 3D Matrix System Applicable to Endothelial Tube Formation Assay Cristina Galindo-Pumariño1, Alberto Herrera2, Alberto Muñoz3, Alfredo Carrato1, Mercedes Herrera4, Cristina Peña1 1Medical Oncology Department, Instituto Ramón y Cajal de Investigación Sanitaria (IRYCIS), CIBERONC, 2Department of Medical Oncology, Hospital Universitario Puerta de Hierro de Majadahonda, 3Instituto de Investigaciones Biomédicas Alberto Sols, Consejo Superior de Investigaciones Científicas - Universidad Autónoma de Madrid, CIBERONC, 4Department of Oncology & Pathology, Karolinska Institutet The aim of this method is to obtain fibroblast-derived 3D matrices as a natural scaffold for subsequent cellular assays. Fibroblasts are seeded in a pre-treated culture plate and stimulated with ascorbic acid for matrix generation. Matrices are decellularized and blocked to culture relevant cells (e.g., endothelial cells). Biochemistry Large-scale Production of Recombinant RNAs on a Circular Scaffold Using a Viroid-derived System in Escherichia coli Teresa Cordero1, Verónica Aragonés1, José-Antonio Daròs1 1Instituto de Biología Molecular y Celular de Plantas, Consejo Superior de Investigaciones Científicas-Universitat Politècnica de València, Spain Here, we present a protocol to produce large amounts of recombinant RNA in Escherichia coli by co-expressing a chimeric RNA that contains the RNA of interest in a viroid scaffold and a plant tRNA ligase. The main product is a circular molecule that facilitates purification to homogeneity. Neuroscience Fluorescence and Bioluminescence Imaging of Subcellular Ca2+ in Aged Hippocampal Neurons María Calvo-Rodríguez1, Carlos Villalobos1, Lucía Nuñez2 1Instituto de Biologìa y Genética Molecular, Consejo Superior de Investigaciones Cientìficas, 2Departamento de Bioquìmica y Biologìa Molecular y Fisiologìa, Universidad de Valladolid Intracellular Ca2+ remodeling in aging may contribute to excitotoxicity and neuron damage, processes mediated by Ca2+ overload. We aimed at investigating Ca2+ remodeling in the aging brain using fluorescence and bioluminescence imaging of cytosolic and mitochondrial Ca2+ in long-term cultures of rat hippocampal neurons, a model of neuronal aging.
