Health Research Institute of the Balearic Islands (IdISBa) 3 articles published in JoVE Immunology and Infection Induction of Periodontitis via a Combination of Ligature and Lipopolysaccharide Injection in a Rat Model Marta Munar-Bestard1,2, Oscar Villa1, Maria del Mar Ferrà-Cañellas1,2,3, Joana Maria Ramis1,2,4,5, Marta Monjo1,2,4,5 1Group of Cell Therapy and Tissue Engineering, Research Institute on Health Sciences (IUNICS), University of the Balearic Islands (UIB), 2Balearic Islands Health Research Institute (IdISBa), 3 In this study, a rat model of induction of periodontitis is presented via a combination of retentive ligature and repetitive injections of lipopolysaccharide derived from Porphyromonas gingivalis, over 14 days around the first maxillary molars. The ligation and LPS injection techniques were effective in inducing peridontitis, resulting in alveolar bone loss and inflammation. Bioengineering Labeling of Extracellular Vesicles for Monitoring Migration and Uptake in Cartilage Explants Maria Antònia Forteza-Genestra1,2, Miquel Antich-Rosselló1,2, Francisco Gabriel Ortega1,2, Guillem Ramis-Munar3, Javier Calvo1,2,4, Antoni Gayà1,2,4, Marta Monjo1,2, Joana Maria Ramis1,2 1Cell Therapy and Tissue Engineering Group, Research Institute on Health Sciences (IUNICS), University of the Balearic Islands, 2Health Research Institute of the Balearic Islands (IdISBa), 3Cellomics Unit, Institut Universitari d'Investigació en Ciències de la Salut (IUNICS), Universitat de les Illes Balears, 4Fundació Banc de Sang i Teixits de les Illes Balears (FBSTIB) Here, we present a protocol to label platelet lysate-derived extracellular vesicles to monitor their migration and uptake in cartilage explants used as a model for osteoarthritis. Bioengineering Platelet-Derived Extracellular Vesicle Functionalization of Ti Implants Miquel Antich-Rosselló1,2, Maria Antònia Forteza-Genestra1,2, Javier Calvo1,2,3, Antoni Gayà1,2,3, Marta Monjo1,2,4, Joana Maria Ramis1,2,4 1Cell Therapy and Tissue Engineering Group, Research Institute on Health Sciences (IUNICS), University of the Balearic Islands, 2Health Research Institute of the Balearic Islands (IdISBa), 3Fundació Banc de Sang i Teixits de les Illes Balears (FBSTIB), 4Department of Fundamental Biology and Health Sciences, University of the Balearic Islands Here, we present a method for the isolation of Extracellular Vesicles (EVs) derived from the platelet lysates (PL) and their use for coating titanium (Ti) implant surfaces. We describe the drop casting coating method, the EVs release profile from the surfaces, and in vitro biocompatibility of EVs coated Ti surfaces.
