University of Sussex 4 articles published in JoVE Biochemistry Malachite Green Assay for the Discovery of Heat-Shock Protein 90 Inhibitors Sayan Dutta Gupta1, Dae-Geun Song1, SueJung Lee1, Jae Wook Lee1, Jin-Soo Park1, Chrisostomos Prodromou2, Cheol-Ho Pan1 1Gangneung Institute of Natural Products, Korea Institute of Science and Technology (KIST), 2School of Life Sciences, University of Sussex The malachite green assay protocol is a simple and cost-effective method to discover heat shock protein 90 (Hsp90) suppressors, as well as other inhibitor compounds against ATP-dependent enzymes. Behavior Visual Classical Conditioning in Wood Ants A. Sofia D. Fernandes1,2,3, C. L. Buckley1,3, J. E. Niven2,3 1Department of Informatics, University of Sussex, 2School of Life Sciences, University of Sussex, 3Centre for Computational Neuroscience and Robotics, University of Sussex We present a protocol for the classical conditioning of harnessed ants that permits researchers to study visual learning and memory formation at a level of analysis not possible with freely moving individuals. Engineering Generation and Coherent Control of Pulsed Quantum Frequency Combs Benjamin MacLellan*1, Piotr Roztocki*1, Michael Kues1,2, Christian Reimer1, Luis Romero Cortés1, Yanbing Zhang1, Stefania Sciara1,3, Benjamin Wetzel1,4, Alfonso Cino3, Sai T. Chu5, Brent E. Little6, David J. Moss7, Lucia Caspani8, José Azaña1, Roberto Morandotti1,9,10 1Institut National de la Recherche Scientifique - Centre Énergie, Matériaux et Télécommunications (INRS-EMT), 2School of Engineering, University of Glasgow, 3Department of Energy, Information Engineering and Mathematical Models, University of Palermo, 4School of Mathematical and Physical Sciences, University of Sussex, 5Department of Physics and Material Science, City University of Hong Kong, 6State Key Laboratory of Transient Optics and Photonics, Xi'an Institute of Optics and Precision Mechanics, Chinese Academy of Science, 7Centre for Micro Photonics, Swinburne University of Technology, 8Institute of Photonics, Department of Physics, University of Strathclyde, 9Institute of Fundamental and Frontier Sciences, University of Electronic Science and Technology of China, 10National Research University of Information Technologies, Mechanics and Optics A protocol is presented for the practical generation and coherent manipulation of high-dimensional frequency-bin entangled photon states using integrated micro-cavities and standard telecommunications components, respectively. Neuroscience Determination of Photoreceptor Cell Spectral Sensitivity in an Insect Model from In Vivo Intracellular Recordings Kyle J. McCulloch1, Daniel Osorio2, Adriana D. Briscoe1 1Department of Ecology and Evolutionary Biology, University of California, Irvine, 2School of Life Sciences, University of Sussex The electrophysiological technique of intracellular recording is demonstrated and used to determine spectral sensitivities of single photoreceptor cells in the compound eye of a butterfly.
