International Institute of Molecular and Cell Biology in Warsaw 1 article published in JoVE Biochemistry High-Throughput Image-Based Quantification of Mitochondrial DNA Synthesis and Distribution Lukasz S. Borowski1,2, Karolina Kasztelan2,3, Jolanta Czerwinska-Kostrzewska1,2, Roman J. Szczesny2 1Faculty of Biology, Institute of Genetics and Biotechnology, University of Warsaw, 2Institute of Biochemistry and Biophysics, Polish Academy of Sciences, 3International Institute of Molecular and Cell Biology in Warsaw A procedure for studying the dynamics of mitochondrial DNA (mtDNA) metabolism in cells using a multi-well plate format and automated immunofluorescence imaging to detect and quantify mtDNA synthesis and distribution is described. This can be further used to investigate the effects of various inhibitors, cellular stresses, and gene silencing on mtDNA metabolism.
