University of Warsaw View Institution's Website 3 articles published in JoVE Biochemistry High-Throughput Image-Based Quantification of Mitochondrial DNA Synthesis and Distribution Lukasz S. Borowski1,2, Karolina Kasztelan2,3, Jolanta Czerwinska-Kostrzewska1,2, Roman J. Szczesny2 1Faculty of Biology, Institute of Genetics and Biotechnology, University of Warsaw, 2Institute of Biochemistry and Biophysics, Polish Academy of Sciences, 3International Institute of Molecular and Cell Biology in Warsaw A procedure for studying the dynamics of mitochondrial DNA (mtDNA) metabolism in cells using a multi-well plate format and automated immunofluorescence imaging to detect and quantify mtDNA synthesis and distribution is described. This can be further used to investigate the effects of various inhibitors, cellular stresses, and gene silencing on mtDNA metabolism. Engineering Free-form Light Actuators — Fabrication and Control of Actuation in Microscopic Scale Hao Zeng1, Piotr Wasylczyk2, Camilla Parmeggiani1,3, Daniele Martella1,4, Diederik Sybolt Wiersma1 1European Labratory for Non-Linear Spectroscopy, University of Florence, 2Institute of Experimental Physics, Faculty of Physics, University of Warsaw, 3CNR-INO, 4Chemistry Department, University of Florence Here, we fabricate 3D polymeric micro/nano structures in which both the shape and the molecular alignment can be engineered with nanometer scale accuracy by the use of direct laser writing. Light induced deformation of several types of liquid crystalline elastomer microstructures can be controlled in the microscopic scale. Biology Visualization of DNA Replication in the Vertebrate Model System DT40 using the DNA Fiber Technique Rebekka A.V. Schwab1, Wojciech Niedzwiedz1,2 1Department of Molecular Oncology, Weatherall Institute of Molecular Medicine, University of Oxford, 2Institute of Genetics and Biotechnology, Faculty of Biology, University of Warsaw DT40, a model vertebrate genetic system, provides a powerful tool to analyze protein function. Here we describe a simple method that allows qualitative analysis of parameters that influence DNA synthesis during the S-phase in DT40 cells at the single molecule level.
