Faculdade de Medicina da Universidade de Lisboa 4 articles published in JoVE Medicine Urethral Stricture Induction Followed by Buccal Mucosa Graft Urethroplasty in a Rat Model Pedro S. de Oliveira1,2, Filipe Rocha1, Inês Sofia Vala1, Paula de Oliveira1, Augusto Ministro1,3, Susana Constantino Rosa Santos1 1Cardiovascular Center of the University of Lisbon (CCUL@RISE), Faculdade de Medicina da Universidade de Lisboa, 2Department of Urology, Centro Hospitalar Universitário Lisboa Norte, 3Department of Vascular Surgery, Centro Hospitalar Universitário Lisboa Norte In the present protocol, a urethral stricture induction was developed in Wistar rats, followed by urethral reconstruction with a buccal mucosa graft. A retrograde urethrogram and laser Doppler assessment were performed, validating urethral reconstruction (after stricture formation) and graft placement. Neuroscience Using the MouseWalker to Quantify Locomotor Dysfunction in a Mouse Model of Spinal Cord Injury Ana Filipa Isidro1, Alexandra M. Medeiros2, Isaura Martins1, Dalila Neves-Silva1, Leonor Saúde1,3, César S. Mendes2 1Instituto de Medicina Molecular João Lobo Antunes, Faculdade de Medicina da Universidade de Lisboa, 2iNOVA4Health, NOVA Medical School|Faculdade de Ciências Médicas, NMS|FCM, Universidade Nova de Lisboa, 3Instituto de Histologia e Biologia do Desenvolvimento, Faculdade de Medicina da Universidade de Lisboa An experimental pipeline to quantitatively describe the locomotor pattern of freely walking mice using the MouseWalker (MW) toolbox is provided, ranging from initial video recordings and tracking to post-quantification analysis. A spinal cord contusion injury model in mice is employed to demonstrate the usefulness of the MW system. Biology Live-Cell Imaging of Transcriptional Activity at DNA Double-Strand Breaks Madalena R. de Almeida1, Eduardo Gameiro1, Sérgio F. de Almeida1, Robert M. Martin1 1Instituto de Medicina Molecular João Lobo Antunes, Faculdade de Medicina da Universidade de Lisboa This protocol presents a new reporter gene system and the experimental setup to detect transcription at DNA double-strand breaks with single-molecule sensitivity. Neuroscience A Model of Epileptogenesis in Rhinal Cortex-Hippocampus Organotypic Slice Cultures Cláudia A. Valente1,2, Francisco J. Meda*1,2, Mafalda Carvalho*1,2, Ana M. Sebastião1,2 1Instituto de Farmacologia e Neurociências, Faculdade de Medicina da Universidade de Lisboa, 2Instituto de Medicina Molecular João Lobo Antunes, Faculdade de Medicina da Universidade de Lisboa Here, we describe the preparation of rhinal cortex-hippocampus organotypic slices. Under a gradual and controlled deprivation of serum, these slices depict evolving epileptic-like events and can be considered an ex vivo model of epileptogenesis. This system represents an excellent tool for monitoring the dynamics of spontaneous activity, as well as for assessing the progression of neuroinflammatory features throughout the course of epileptogenesis.
