Schepens Eye Research Institute of Mass. Eye and Ear 2 articles published in JoVE Biology Real-Time Analysis of Bioenergetics in Primary Human Retinal Pigment Epithelial Cells Using High-Resolution Respirometry Tessa C. Fitch*1,2, Scott I. Frank*1, Yutong Kelly Li1, Magali Saint-Geniez1,2, Leo A. Kim1,2, Daisy Y. Shu1,2 1Schepens Eye Research Institute of Mass. Eye and Ear, 2Department of Ophthalmology, Harvard Medical School The metabolic status of human retinal pigment epithelial cells (H-RPE) reflects their health and function. Presented here is an optimized protocol for examining the real-time metabolic flux of H-RPE using high-resolution respirometry. Developmental Biology Generation of Retinal Organoids from Healthy and Retinal Disease-Specific Human-Induced Pluripotent Stem Cells Sudipta Mahato*1,2, Trupti Agrawal*1,2, Divya Pidishetty*1,2, Savitri Maddileti1, Vinay Kumar Pulimamidi1,3, Indumathi Mariappan1 1Centre for Ocular Regeneration, Prof. Brien Holden Eye Research Centre, LV Prasad Eye Institute, 2Manipal Academy of Higher Education, 3Schepens Eye Research Institute, Massachusetts Eye and Ear, Harvard Medical School This protocol describes an efficient method of differentiating hiPSCs into eye field clusters and generating neuro-retinal organoids using simplified culture conditions involving both adherent and suspension culture systems. Other ocular cell types, such as the RPE and corneal epithelium, can also be isolated from mature eye fields in retinal cultures.
