SUNY Downstate Medical Center 4 articles published in JoVE Neuroscience In Vivo Fiber-Coupled Pre-Clinical Confocal Laser-scanning Endomicroscopy (pCLE) of Hippocampal Capillaries in Awake Mice Rocio Leal-Campanario1,2, Susana Martinez-Conde2,3, Stephen L. Macknik2,3 1División de Neurociencias, Universidad Pablo de Olavide, 2Barrow Neurological Institute, 3Ophthalmology, Neurology, and Physiology/Pharmacology, SUNY Downstate Medical Center Whereas multiphoton imaging is only effective at limited depths from the tissue’s surface, it is possible to achieve 3 µm resolution imaging at any depth via pCLE. Here, we present a protocol to conduct pCLE imaging to measure microvascular dynamics in the hippocampus of ictal and wild-type mice. Medicine An In Vitro Approach to Photodynamic Therapy Evan Austin1,2, Jared Jagdeo1,2,3 1Department of Dermatology, University of California, Davis, 2Dermatology Service, Sacramento VA Medical Center, 3Department of Dermatology, State University of New York, Downstate Medical Center Photodynamic therapy (PDT) is a medical procedure that involves incubation of an exogenously applied photosensitizer (PS) followed by visible light photoactivation to induce apoptosis. We present an in vitro PDT protocol designed to simulate PDT that may be used to study differences in PS incubation and light treatment parameters. Neuroscience Rapid and Specific Immunomagnetic Isolation of Mouse Primary Oligodendrocytes Rafael E. Flores-Obando1, Mona M. Freidin2, Charles K. Abrams2 1Program in Molecular and Cellular Biology, State University of New York Downstate Medical Center, 2Department of Neurology and Rehabilitation, University of Illinois at Chicago We describe the immunomagnetic isolation of primary mouse oligodendrocytes, which allows the rapid and specific isolation of the cells for in vitro culture. Neuroscience Real-time Iontophoresis with Tetramethylammonium to Quantify Volume Fraction and Tortuosity of Brain Extracellular Space John Odackal*1, Robert Colbourn*2,3, Namrita Jain Odackal4, Lian Tao5, Charles Nicholson5, Sabina Hrabetova2 1Department of Medicine, University of Virginia, 2Department of Cell Biology, SUNY Downstate Medical Center, 3Neural and Behavioral Science Graduate Program, SUNY Downstate Medical Center, 4Division of Neonatology, University of Virginia, 5Department of Neuroscience and Physiology, New York University School of Medicine This protocol describes real-time iontophoresis, a method that measures physical parameters of the extracellular space (ECS) of living brains. The diffusion of an inert molecule released into the ECS is used to calculate the ECS volume fraction and tortuosity. It is ideal for studying acute reversible changes to brain ECS.
