Federal University of Parana 3 articles published in JoVE Environment A 3D Culture Method of Spheroids of Embryonic and Liver Zebrafish Cell Lines Irisdoris Rodrigues de Souza1, Andrezza Di Pietro Micali Canavez2, Desiree Cigaran Schuck2, Viviana Stephanie Costa Gagosian1, Isisdoris Rodrigues de Souza1, Natália de Albuquerque Vita2, Edvaldo da Silva Trindade3, Marta Margarete Cestari4, Marcio Lorencini2, Daniela Morais Leme4 1Graduate Program in Genetics, Department of Genetics, Federal University of Paraná (UFPR), 2Safety of Product Department, Grupo Boticário, 3Department of Cell Biology, Federal University of Paraná (UFPR), 4Department of Genetics, Federal University of Paraná (UFPR) Here, we present an effective, easy, and fast 3D culture protocol for the formation of spheroids of two zebrafish (Danio rerio) cell lines: ZEM2S (embryo) and ZFL (normal hepatocyte). Biochemistry An In-House-Built and Light-Emitting-Diode-Based Photodynamic Therapy Device for Enhancing Verteporfin Cytotoxicity in a 2D Cell Culture Model Isadora da Silva Zanzarini1, Gustavo Barbosa1, Larissa de Oliveira Prado1, Ingrid Fatima Zattoni1, Gustavo Da Paz1, Ademir Luiz do Prado2,3, Waldemar Volanski2, Marcos Dinís Lavarda3, Fabiane Gomes de Moraes Rego2, Geraldo Picheth2, Vivian Rotuno Moure1, Glaucio Valdameri1 1Graduate Program in Pharmaceutical Sciences, Laboratory of Cancer Drug Resistance, Federal University of Parana, 2Graduate Program in Pharmaceutical Sciences, Federal University of Parana, 3Federal Institute of Parana Here, we describe a novel, simple, and low-cost device to successfully perform in vitro photodynamic therapy (PDT) assays using two-dimensional HeLa cell culture and verteporfin as a photosensitizer. Environment Cytotoxicity Assays with Zebrafish Cell Lines Irisdoris Rodrigues de Souza1, Tainá Wilke Sivek1, Júlia Beatriz Vaz de Oliveira1, Andrezza Di Pietro Micali Canavez2, Natália de Albuquerque Vita2, Desiree Cigaran Schuck2, Isisdoris Rodrigues de Souza1, Marta Margarete Cestari3, Marcio Lorencini2, Daniela Morais Leme3 1Graduate Program in Genetics, Department of Genetics, Federal University of Paraná (UFPR), 2Safety of Product Department, Grupo Boticário, 3Department of Genetics, Federal University of Paraná (UFPR) This protocol presents commonly used cytotoxicity assays (Alamar Blue [AB], CFDA-AM, Neutral Red, and MTT assays) adapted for the assessment of cytotoxicity in zebrafish embryo (ZEM2S) and liver (ZFL) cell lines in 96-well plates.
