Jove Lab Chem

Lab 63: UV-Vis Spectroscopy of Dyes — Materials List

Preparation for 10 Students

• β-Carotene
  40 mg
• Fluorescein
  20 mg
• Indigo dye
  20 mg
• Hexane
  1000 mL
• Dimethylformamide
  500 mL
• Acetone
  250 mL
• Pasteur pipette bulb
  15
• Quartz cuvette with cap (1 cm x 1 cm)
  10
• UV-Vis single-beam spectrophotometer OR Handheld Data Acquistion Device with UV spectrophotometer attachment
  Dependent on lab size
• 50-mL glass volumetric flask with stopper
  11
• 50-mL glass graduated cylinder
  3
• Small glass funnel
  3
• 200-µL micropipette with tips
  1
• 1000-µL micropipette with tips
  1
• Pasteur pipettes
  Dependent on lab size
• 250-mL plastic wash bottle
  Dependent on lab size
• Analytical balance
  1
• Weighing boats
  3
• Spatulas
  3
• Lab wipes
  Dependent on lab size
• Labeling tape
  1 roll
• Labeling pen
  1
• Paraffin film
  1 box
• Deionized water
  Dependent on lab size
• Aluminum foil
  1 roll