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Uso di dispositivi microfluidici misurare la durata della vita e cellulare fenotipi in singole cellule di lievito in erba

Article DOI: 10.3791/55412-v • 09:18 min • March 30th, 2017
March 30th, 2017 •
1,2, 2, 1,3, 2, 2
1The State Key Laboratory for Artificial Microstructures and Mesoscopic Physics, School of Physics, Peking University, 2Department of Biochemistry and Biophysics, University of California, San Francisco, 3Peking-Tsinghua Center for Life Sciences at Center for Quantitative Biology, Academy for Advanced Interdisciplinary Studies, Peking University

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Questo articolo presenta un protocollo ottimizzato per la produzione di chip microfluidici e la configurazione degli esperimenti microfluidici per misurare la durata di vita e fenotipi cellulari delle singole cellule di lievito.

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Biofisica , Microfluidica circuito integrato il lievito l'imaging la durata della vita replicativa live-cell imaging
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