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JoVE Journal Genetics
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Identifiering av cirkulär RNAs med RNA-sekvensering

Article DOI: 10.3791/59981-v • 08:25 min • November 14th, 2019
November 14th, 2019 •
1,2, 1,2, 1,2, 1,2, 2,3, 2,3, 1,2
1Translational Genomic Research Institute, 2Arizona Alzheimer's Consortium, 3Banner Sun Health Research Institute

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Cirkulär RNAs (circRNAs) är icke-kodande RNAs som kan ha roller i transkriptionell reglering och förmedla interaktioner mellan proteiner. Efter bedömning av olika parametrar för byggandet av circRNA sekvenserings bibliotek, ett protokoll sammanställdes utnyttjar Stranded totalt RNA Library beredning med RNase R pre-behandling och presenteras här.

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Genetik cirkulär RNA cirkulär RNA-berikning RNase R RNA-biblioteksberedning nästa generations sekvensering RNA-sekvensering
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