JoVE Journal
Cancer Research
Cancer Research
Se requiere una suscripción a JoVE para ver este contenido.
Capítulos
Resumen
Development of murine models with specific genes mutated in head and neck cancer patients is required for understanding of neoplasia. Here, we present a protocol for in vitro transformation of primary murine tongue cells using an adeno-associated virus-Cas9 system to generate murine HNC cell lines with specific genomic alterations.