Screening of Tobacco Genotypes for <em>Phytophthora nicotianae</em> Resistance

Yutong Liu; Mingming Sun; Zipeng Jiang; Xinfang Wang; Bingguang Xiao; Aiguo Yang; He Meng; Lirui Cheng

doi:10.3791/63054

Cribado de genotipos de tabaco para la resistencia a Phytophthora nicotianae

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Screening of Tobacco Genotypes for Phytophthora nicotianae Resistance

Cribado de genotipos de tabaco para la resistencia a Phytophthora nicotianae

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10:10 min

April 15, 2022

DOI: 10.3791/63054-v

Yutong Liu¹, Mingming Sun¹, Zipeng Jiang¹, Xinfang Wang², Bingguang Xiao³, Aiguo Yang¹, He Meng¹, Lirui Cheng¹

¹Key Laboratory of Tobacco Genetic Improvement and Biotechnology, Tobacco Research Institute,Chinese Academy of Agricultural Sciences, ²Institute of Rural Development,Zhejiang Academy of Agricultural Sciences, ³Yunnan Academy of Tobacco Agricultural Sciences

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Overview

This protocol outlines an efficient method for screening tobacco genotypes for resistance to Phytophthora nicotianae in seedlings, contributing to precision breeding and molecular mechanism research.

Key Study Components

Research Area

Plant resistance to pathogens
Precision breeding methods
Evaluation of disease severity in tobacco

Background

Importance of Phytophthora nicotianae in tobacco cultivation
Resistance mechanisms in tobacco varieties
Use of hydroponic systems for research

Methods Used

Seedling screening for pathogen resistance
Tobacco as the biological system
Assessment of disease severity using a standard grading scale

Main Results

Resistant variety BH showed no symptoms after inoculation, while susceptible variety XHJ exhibited significant disease symptoms.
Mean disease index confirmed BH's resistance and XHJ's susceptibility.
Realtime PCR validated the findings on pathogen biomass.

Conclusions

The study successfully demonstrates a reliable method for identifying resistant genotypes in tobacco.
It highlights the relevance of precision breeding in mitigating disease impact on tobacco crops.

Frequently Asked Questions

What is the significance of this research?

This research aids in developing tobacco strains with enhanced resistance to Phytophthora nicotianae, supporting sustainable agriculture.

How does the screening process work?

The process involves evaluating seedlings for disease symptoms post-inoculation with the pathogen using a standardized scoring system.

What are the implications of this study for breeding programs?

This method provides a practical approach for identifying resistant varieties, which can be integrated into breeding programs to improve crop resilience.

What are the key components of the methods used?

The key components include seed selection, hydroponic transplanting, and standardized symptomatic scoring.

How was disease severity quantified?

Disease severity was quantified using a grading scale ranging from 0 to 9 based on observed symptoms.

Can this protocol be adapted to other plant types?

Yes, while optimized for tobacco, it may be adapted for use in other crops susceptible to similar pathogens.

What technologies support this research?

The research utilizes hydroponic technologies and real-time PCR for pathogen quantification and assessment.

Aquí, se presenta un protocolo para la detección eficiente y precisa de genotipos de tabaco para la resistencia a Phytophthora nicotianae en plántulas. Este es un enfoque práctico para la cría de precisión, así como para la investigación de mecanismos moleculares.

Aquí, se presenta un protocolo para la detección eficiente y precisa de genotipos de tabaco para la resistencia a Phytophthora nicotianae en plántulas. Es práctico para la cría de precisión, así como para la investigación de mecanismos moleculares. Materiales. Obtenga las variedades de tabaco Beinhart1000-1, una selección de Beinhart1000 BH y Xiaohuangjin1025 XHJ del Geneback Nacional a Mediano Plazo del Recurso de Germoplasma de Tabaco de China.

BH es resistente, mientras que XHJ es susceptible a la infección por Phytophthora nicotianae. Plantación de genotipos de tabaco para la evaluación de la resistencia a Phytophthora nicotianae. Mezcle las semillas de tabaco con vermiculita y transmita las semillas suavemente sobre el suelo esterilizado para macetas.

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