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Immunology and Infection

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JoVE Journal Immunology and Infection
Detektion av polyfunktionella T-celler hos barn vaccinerade med japanskt encefalitvaccin via flödescytometritekniken
Collection, Embedding, Sectioning, and Staining of the Mouse Submandibular Salivary Gland for Live Cell Imaging of Cell-Cell Interactions (Video) | JoVE
Journal (Immunology and Infection)
Collection, Embedding, Sectioning, and Staining of the Mouse Submandibular Salivary Gland for Live Cell Imaging of Cell-Cell Interactions (Video) | JoVE
Subcellular Fractionation from Fresh and Frozen Gastrointestinal Specimens
Journal (Immunology and Infection)
Subcellular Fractionation from Fresh and Frozen Gastrointestinal Specimens
 
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Detektion av polyfunktionella T-celler hos barn vaccinerade med japanskt encefalitvaccin via flödescytometritekniken

Article DOI: 10.3791/64671-v • 09:37 min • September 23rd, 2022
September 23rd, 2022 •
*1,2, *1,2,3, 1,2, 1,2, 1,2, 4, 1,2, 1,2
1Beijing Key Laboratory of Pediatric Respiratory Infectious Diseases, Key Laboratory of Major Diseases in Children, Ministry of Education, National Clinical Research Center for Respiratory Diseases, Laboratory of Infection and Virology, Beijing Pediatric Research Institute, Beijing Children’s Hospital, Capital Medical University, National Center for Children’s Health, 2Research Unit of Critical Infection in Children, 2019RU016, Chinese Academy of Medical Sciences, 3Department of Pediatric Rehabilitation, Beijing Boai Hospital, School of Rehabilitation Medicine, Capital Medical University, China Rehabilitation Research Center, 4Center of Excellence (Beijing), Becton Dickinson Medical Devices (Shanghai) Co Ltd
* These authors contributed equally

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Detta protokoll kombinerar ex vivo-stimulering och flödescytometri för att analysera polyfunktionella T-cellprofiler (TPF) i mononukleära celler i perifert blod (PBMC) inom japanskt encefalitvirus (JEV) -vaccinerade barn. Detektionsmetoden och flödescytometrifärgschemat för JEV-specifika TPF testades för att ge en referens för liknande studier.

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Immunologi och infektion utgåva 187 Polyfunktionella T-celler japansk encefalit vaccination flödescytometri
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