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Human Liver Spheroids from Peripheral Blood for Liver Disease Studies

Article DOI: 10.3791/64703-v • 09:51 min • January 27th, 2023
January 27th, 2023 •
1, 2, 2, 1
1ACA CELL Biotech GmbH, 2Departamento Ciencias Biomédicas, Universidad Cardenal Herrera-CEU

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Here we present a non-genetic method to generate human autologous liver spheroids using mononuclear cells isolated from steady-state peripheral blood.

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Human Liver Spheroids Peripheral Blood Liver Disease Studies Autologous Sources Toxicology Cancer Research Drug Discovery BDPC-derived Human Hepatocytes Primary Human Hepatocytes Regenerative Medicine Therapy Acute Liver Failure Hepatoblast KnockOut Serum Replacement Dimethyl Sulfoxide (KSR DMSO) Medium Hepatocyte Maturation Medium Hepatocyte Growth Factor (HGF) Oncostatin M (OCM) Blood-derived Pluripotent Stem Cells (BD-PSCs) Biolaminin Coated Four-well Plate Endodermal Differentiation
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