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Studying the Epithelial Effects of Intestinal Inflammation In Vitro on Established Murine Colonoids (Video) | JoVE

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Journal (Biology)

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Journal (Biology)

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Studying the Epithelial Effects of Intestinal Inflammation In Vitro on Established Murine Colonoids (Video) | JoVE

Epithelial Effects of Intestinal Inflammation on Established Murine Colonoids

Article DOI: 10.3791/64804-v • 01:30 min • June 2nd, 2023

June 2nd, 2023 •

Erin Crawford*¹, Heather L. Mentrup*^2,3, Elizabeth A. Novak^2,3, Kevin P. Mollen^2,3

¹Division of Pediatric Gastroenterology, Hepatology and Nutrition, UPMC Children’s Hospital of Pittsburgh, ²Division of Pediatric Surgery, UPMC Children’s Hospital of Pittsburgh, ³Department of Surgery, University of Pittsburgh School of Medicine

* These authors contributed equally

Tags

Epithelial Effects Intestinal Inflammation In Vitro Murine Colonoids Conventional Colonoid Culture System Intestinal Physiology Inflamed Disease State Barrier Function Inflammatory Bowel Disease Stem Cell Physiology Inflammatory Mediators Intestinal Epithelium 2D Monolayer System Human Colonoid Culture System Diseases Dynamic Cell Layer Host And External Environment Microbial And Immune Populations Intestinal Immune Response Disruption Of Epithelial Barrier Therapeutic Targeting

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