//Optical Disector Grid.ijm
//simple imageJ implimentation of an optical disector grid for stereological counting
//code written by Christopher S Ward - 2013 for use in ImageJ/FIJI (Wayne Rasband)
//the code is distributed free of use, acknowledgements/citations appreciated if you use/modify the code
//grid is created in center of image and is sized in pixels (modify code below)
//should be compatable with "Cell Counter" plug-in

// user defined parameters
 usergrid=212.5;
 imagex=1428.25;
 imagey=2313.25;
 
//overlay grid
 Overlay.remove;
 setColor("red");
 Overlay.drawLine(imagex-usergrid*0.5,imagey-usergrid*1.5,imagex-usergrid*0.5,imagey+usergrid*0.5);
 Overlay.drawLine(imagex-usergrid*0.5,imagey+usergrid*0.5,imagex+usergrid*0.5,imagey+usergrid*0.5);
 Overlay.drawLine(imagex+usergrid*0.5,imagey+usergrid*0.5,imagex+usergrid*0.5,imagey+usergrid*1.5);
 Overlay.add;
 setColor("green");
 Overlay.drawLine(imagex-usergrid*0.5,imagey-usergrid*0.5,imagex+usergrid*0.5,imagey-usergrid*0.5)
 Overlay.drawLine(imagex+usergrid*0.5,imagey-usergrid*0.5,imagex+usergrid*0.5,imagey+usergrid*0.5)
 Overlay.add
 Overlay.show