Date: 2021-09-22
Submitted by: Isabelle Sirois
Analysis type: Class II
MHC CLASS II HUMAN JY
MHC_CLASS_II_FROM_JY_-_L243_ANTIBODY: Alleles: DRB1_0404, DRB1_1301
Species: HUMAN - JY
# of cells: 100 M
Lysis buffer: 0.5% CHAPS
Type of beads: 80 mg Sepharose CNBr-activated beads
Antibody: L243 - 2 mg
MHC-ligand complex elution buffer: 1% TFA
Peptide elution buffer: 28% ACN/0.1% TFA
Type of MS/MS: ORBITRAP ECLIPSE TRIBID MASS SPECTROMETER
Peptide identification software: PEAKS PRO V10.6
Peptide FDR: 1%
- LF Score: fraction of peptides between 9 and 22 mers. - BF Score: fraction of peptides between 9 and 22 mers which are predicted to be strong or weak binders.
Sample | Total peptides | Peptides between 9-22 mers | LF Score | BF Score |
---|---|---|---|---|
MHC_CLASS_II_FROM_JY_-_L243_ANTIBODY | 1707 | 1614 | 0.95 | 0.7 |
Peptide Length Distribution (maximum of 30 mers)
NetMHCIIpan eluted ligand predictions made for all peptides between 9 & 22 mers, inclusive. - Percent rank cutoffs for strong and weak binders: 2.0 and 10.0. - Percentages are calculated across rows (i.e. percentage of total peptides for a respective sample).
Sample | Total peptides | Allele | Strong binders | Weak binders | Non-binders |
---|---|---|---|---|---|
MHC_CLASS_II_FROM_JY_-_L243_ANTIBODY |
1614 | DRB1_0404 | 627 (38.8%) | 352 (21.8%) | 635 (39.3%) |
DRB1_1301 | 125 (7.7%) | 338 (20.9%) | 1151 (71.3%) |
NetMHCIIpan eluted ligand predictions made for all peptides between 9 & 22 mers, inclusive. Approximate color legend (detailed mapping shown next to heatmaps):
Predicted strong binders (%rank <= 2.0)
Predicted weak binders (2.0 < %rank <= 10.0)
Predicted non-binders
- - # of peptides in sample
Clustering performed with all peptides between 9 & 22 mers, inclusive.
- Percentages represent the percentage of peptides in a given group predicted to strongly bind the indicated allele.
Polar
Neutral
Basic
Acidic
Hydrophobic
MHC_CLASS_II_FROM_JY_-_L243_ANTIBODY (peptides clustered: 1614, outliers: 36)
Peptides in group: 270
DRB1_0404: 11%,
DRB1_1301: 20%Peptides in group: 603
DRB1_1301: 8%
Peptides in group: 265
DRB1_1301: 7%
Peptides in group: 440
DRB1_1301: 1%
Peptides: 966
Peptides: 447
Peptides: 246
Peptides: 202