Single-cell analysis for gene: MMP1 

Input object: H:/糖尿病足--DFU-Control/GSE68183-GSE80178合并建模/线粒体氧化膜裂死亡/9.单细胞分析/GSE165816--实验集/02.SingleR_output/biofsci_SingleR_annotated.rds 
Number of cells: 69944 
Number of genes: 22889 

Cell type column: SingleR_label 
Group column: Group 
Sample column: orig.ident 

Control group: Control 
Disease group: DFU 

MMP1 expression summary:
   Min. 1st Qu.  Median    Mean 3rd Qu.    Max. 
  0.000   0.000   0.000   0.112   0.000   6.237 

MMP1 positive cells:

MMP1- MMP1+ 
66145  3799 

Top MMP1-expressing cell types:
# A tibble: 10 × 4
   CellType_for_MMP1 mean_MMP1 positive_ratio n_cells
   <fct>                 <dbl>          <dbl>   <int>
 1 Fibroblasts         0.181          0.0788    39801
 2 B cells             0.0497         0.0381     1128
 3 Monocytes           0.0366         0.0324     3241
 4 CD8+ T cells        0.0286         0.0193     5377
 5 Keratinocytes       0.0209         0.0318     7431
 6 Macrophages         0.0159         0.0136     2206
 7 Endothelial cells   0.0141         0.0157     7498
 8 Epithelial cells    0.0116         0.0142     1759
 9 HSC                 0.00480        0.00294     680
10 Neurons             0              0           823

Cell type-specific DFU vs Control statistics:
# A tibble: 10 × 11
   CellType_for_MMP1 Control_n_cells Disease_n_cells Control_mean Disease_mean Control_median Disease_median logFC_Disease_vs_Control
   <fct>                       <int>           <int>        <dbl>        <dbl>          <dbl>          <dbl>                    <dbl>
 1 Keratinocytes                3913            3518     0.000160       0.0440              0              0                  0.0438 
 2 Fibroblasts                 16381           23420     0.154          0.199               0              0                  0.0446 
 3 Endothelial cells            3449            4049     0.00181        0.0246              0              0                  0.0228 
 4 B cells                       229             899     0.169          0.0192              0              0                 -0.150  
 5 Epithelial cells             1072             687     0              0.0297              0              0                  0.0297 
 6 CD8+ T cells                 1387            3990     0.0562         0.0190              0              0                 -0.0372 
 7 HSC                           508             172     0              0.0190              0              0                  0.0190 
 8 Macrophages                   403            1803     0.0171         0.0156              0              0                 -0.00151
 9 Monocytes                     669            2572     0.0334         0.0374              0              0                  0.00404
10 Neurons                       548             275     0              0                   0              0                  0      
# ℹ 3 more variables: p_value <dbl>, p_adj_BH <dbl>, significance <chr>

Pseudobulk statistics:
# A tibble: 10 × 9
   CellType_for_MMP1 Control_n_samples Disease_n_samples Control_mean Disease_mean logFC_Disease_vs_Cont…¹   p_value p_adj_BH significance
   <fct>                         <int>             <int>        <dbl>        <dbl>                   <dbl>     <dbl>    <dbl> <chr>       
 1 Keratinocytes                     9                14    0.0000866       0.0448                 0.0447    0.00386   0.0214 **          
 2 Fibroblasts                      10                14    0.146           0.224                  0.0786    0.00569   0.0214 **          
 3 Endothelial cells                10                14    0.0118          0.0355                 0.0237    0.00713   0.0214 **          
 4 Epithelial cells                  9                14    0               0.0193                 0.0193    0.0953    0.198  ns          
 5 Macrophages                      10                13    0.0119          0.0266                 0.0147    0.110     0.198  ns          
 6 CD8+ T cells                     10                14    0.0141          0.0187                 0.00457   0.248     0.314  ns          
 7 HSC                               8                12    0               0.0408                 0.0408    0.267     0.314  ns          
 8 Monocytes                        10                14    0.0125          0.0246                 0.0121    0.280     0.314  ns          
 9 B cells                           2                13    0.0855          0.0170                -0.0684    0.502     0.502  ns          
10 Neurons                           9                11    0               0                      0       NaN       NaN      NA          
# ℹ abbreviated name: ¹​logFC_Disease_vs_Control

Correlation with functional module scores:
                                  Score Spearman_rho       p_value      p_adj_BH
rho               ECM_remodeling_Score1   0.24022938  0.000000e+00  0.000000e+00
rho3            Oxidative_stress_Score1   0.16090863  0.000000e+00  0.000000e+00
rho4             Senescence_SASP_Score1   0.27413452  0.000000e+00  0.000000e+00
rho5                 Ferroptosis_Score1   0.19705894  0.000000e+00  0.000000e+00
rho6       Fibroblast_activation_Score1   0.16989125  0.000000e+00  0.000000e+00
rho1                Inflammation_Score1   0.13324292 1.970653e-274 3.284422e-274
rho8       Macrophage_activation_Score1   0.09540507 4.225120e-141 6.035885e-141
rho2                Angiogenesis_Score1   0.06865460  7.687388e-74  9.609235e-74
rho7 Keratinocyte_wound_response_Score1  -0.04175511  2.252464e-28  2.502738e-28
rho9     Endothelial_dysfunction_Score1   0.04010488  2.660723e-26  2.660723e-26

