Pairing is based on explicit author confirmation: NC/DFU, NC1/DFU1, NC2/DFU2, NC3/DFU3.
WB uses three corresponding rows confirmed by authors. No claim that all four qPCR patients also had WB is made.
Five ACTB and five MMP1 wells are averaged separately per specimen. Paired qPCR test uses DeltaCq, not five well-level fold values.
Panel A is preserved from the supplied representative blot, cropped only to isolate the original panel, without intensity adjustment.
Original lane-to-quantification and background subtraction records remain incomplete. Small-n results are preliminary.
