Primary Culture of Adult Rat Heart Myocytes

Xianghua Xu; Henry M. Colecraft

doi:10.3791/1308

Journal

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Biologie

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Primary Culture of Adult Rat Heart Myocytes

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Biologie

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Journal JoVE Biologie

Primary Culture of Adult Rat Heart Myocytes

DOI:

10.3791/1308-v

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11:44 min

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June 16, 2009

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Xianghua Xu, Henry M. Colecraft²

¹Department of Physiology and Cellular Biophysics,Columbia University, ²Department of Pharmacology,Columbia University

Chapitres

00:04Titre
00:52Introduction
01:18Preparation for Surgery
04:06The Rat Heart Dissection
05:05Myocytes Isolation
10:15Myocyte Cell Culture
10:56Representative Outcome: Cultured Myocytes
11:20Conclusion

Summary

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In this paper, we described a typical way to isolate and culture adult rat heart myocytes. Collagenase and protease are used to digest and isolate single myocytes. Myocytes cultured follow this protocol meet most experiment requirements.

Primary Culture of Adult Rat Heart Myocytes

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Chapitres

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