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Omprogrammering Mänskliga somatiska celler in i inducerade pluripotenta stamceller (iPSCs) Använda retroviral vektor med GFP
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Omprogrammering Mänskliga somatiska celler in i inducerade pluripotenta stamceller (iPSCs) Använda retroviral vektor med GFP

Article DOI: 10.3791/3804-v • 08:25 min • April 3rd, 2012
April 3rd, 2012 •
1, 1, 1
1Yale Stem Cell Center, Department of Genetics, Yale School of Medicine

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En metod för att generera humana inducerade pluripotenta stamceller (iPSCs) via retrovirus-medierad ektopiskt uttryck av Oct4 är SOX2, KLF4 och MYC beskrivits. Ett praktiskt sätt att identifiera de mänskliga IPSC kolonier som bygger på GFP-uttryck diskuteras också.

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Stem Cell Biology Human iPS-celler omprogrammering Retrovirusvektorer och Pluripotens
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