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Generering av en gen-forstyrret Streptococcus mutans belastning uten Gene kloning

Article DOI: 10.3791/56319-v • 12:06 min • October 23rd, 2017
October 23rd, 2017 •
1, 1, 1,2,3, 1
1Department of Translational Research, Tsurumi University School of Dental Medicine, 2Endowed Department of International Oral Health Science (affiliated with Department of Translational Research), Tsurumi University School of Dental Medicine, 3Cariology and Operative Dentistry, Graduate School of Medical and Dental Sciences, Tokyo Medical and Dental University

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Vi beskriver en lettvinte, rask og relativt rimelig metode for generering av gen-forstyrret Streptococcus mutans stammer; Denne teknikken kan tilpasses for generering av gen-forstyrret stammer av ulike arter.

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Genetikk problemet 128 Gene avbrudd DNA konstruksjon polymerasekjedereaksjons primer design antibiotikaresistens markør genet Streptococcus mutans electroporation homologe rekombinasjon
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