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Chemistry

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JoVE Journal Chemistry
Væsentlige Metal udbredelse i Gram-negative bakterier
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Væsentlige Metal udbredelse i Gram-negative bakterier: X-ray fluorescens, radioisotoper, og celle fraktionering

Article DOI: 10.3791/57169-v • 10:34 min • February 1st, 2018
February 1st, 2018 •
1, 2, 2, 3, 2, 4
1Graduate Biomedical Sciences Microbiology Theme, University of Alabama at Birmingham, 2Department of Radiology, University of Alabama at Birmingham, 3Office of the Provost, University of Alabama at Birmingham, 4Department of Pharmacology and Toxicology, University of Alabama at Birmingham

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En protokol til udvinding af en periplasmic overgang metal anstandsdame i forbindelse med sine indfødte bindende partnere og Biofysisk karakterisering af substrat indholdet af X-ray fluorescens og radiometal optagelse er præsenteret.

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Kemi sag 132 fraktionering Periplasm X-Ray fluorescens overgang Metal substrat-bindende Protein (SBP) YfeA Yersinia pestis pest Radiotracer radioaktivitet
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