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Isolation of Myoepithelial Cells from Adult Murine Lacrimal and Submandibular Glands
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Isolation of Myoepithelial Cells from Adult Murine Lacrimal and Submandibular Glands

Article DOI: 10.3791/59602-v • 07:15 min • June 11th, 2019
June 11th, 2019 •
1, 1, 1
1Department of Molecular Medicine, The Scripps Research Institute

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The lacrimal gland (LG) has two cell types expressing α-smooth muscle actin (αSMA): myoepithelial cells (MECs) and pericytes. MECs are of ectodermal origin, found in many glandular tissues, while pericytes are vascular smooth muscle cells of endodermal origin. This protocol isolates MECs and pericytes from murine LGs.

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Isolation Myoepithelial Cells Adult Murine Lacrimal Gland Submandibular Gland Smooth Muscle Cell Lines Pericytes Genetic Labeling Cell Surface Markers Purification Comparison Function Regenerative Abilities Diseased Cells Gene Expression Studies Exocrine Glands Mammary Gland Salivary Gland Pancreas Tamoxifen-inducible Alpha SMA Driven Reporter Mice Injection Lacrimal Gland Collection Connective Tissue Scissors
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