Fabrication of Ti3C2 MXene Microelectrode Arrays for In Vivo Neural Recording

Nicolette Driscoll; Kathleen Maleski; Andrew  G. Richardson; Brendan Murphy; Babak Anasori; Timothy  H. Lucas; Yury Gogotsi; Flavia Vitale

doi:10.3791/60741

Fabrication de Ti3C2 MXene Microelectrode Arrays pour In Vivo Neur...

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JoVE Journal Bioengineering

Fabrication of Ti₃C₂ MXene Microelectrode Arrays for In Vivo Neural Recording

Fabrication de Ti3C2 MXene Microelectrode Arrays pour In Vivo Neural Recording

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09:58 min

February 12, 2020

DOI: 10.3791/60741-v

Nicolette Driscoll^1,2,3, Kathleen Maleski^4,5, Andrew G. Richardson^2,6, Brendan Murphy^1,2,3, Babak Anasori^4,5, Timothy H. Lucas^2,6, Yury Gogotsi^4,5, Flavia Vitale^1,2,3,7,8

¹Department of Bioengineering,University of Pennsylvania, ²Center for Neuroengineering and Therapeutics,University of Pennsylvania, ³Corporal Michael J. Crescenz Veterans Affairs Medical Center, ⁴Department of Materials Science and Engineering,Drexel University, ⁵A.J. Drexel Nanomaterials Institute,Drexel University, ⁶Department of Neurosurgery,University of Pennsylvania, ⁷Department of Neurology,University of Pennsylvania, ⁸Department of Physical Medicine and Rehabilitation

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Overview

This article describes a method for fabricating Ti₃C₂ MXene microelectrode arrays and their application in in vivo neural recording. The technique allows for precise micro-patterning of MXene films while maintaining their integrity.

Key Study Components

Area of Science

Neuroscience
Materials Science
Electrophysiology

Background

MXenes are a class of two-dimensional materials with promising electrical properties.
Ti₃C₂ MXene exhibits high metallic conductivity.
Microelectrode arrays are essential for advanced neural recording techniques.
Precise fabrication methods are crucial for the performance of neural interfaces.

Purpose of Study

To develop a reliable method for creating Ti₃C₂ MXene microelectrode arrays.
To demonstrate the application of these arrays in in vivo neural recording.
To enhance the understanding of MXene properties in neural applications.

Methods Used

Preparation of titanium carbide ink through selective etching.
Micro-patterning of MXene films without damaging the electrodes.
Centrifugation and washing to achieve desired pH levels.
Intercalation of lithium chloride to modify MXene properties.

Main Results

The synthesized MXene ink demonstrated metallic conductivity above 10,000 Siemens per centimeter.
The microelectrode arrays maintained structural integrity during fabrication.
Successful application in in vivo neural recording was achieved.
The method allows for reproducible and scalable production of MXene electrodes.

Conclusions

This method provides a viable approach for fabricating MXene microelectrode arrays.
MXene films can be effectively used for advanced neural recording applications.
The technique opens new avenues for research in neural interfaces.

Frequently Asked Questions

What are MXenes?

MXenes are a family of two-dimensional transition metal carbides, nitrides, or carbonitrides known for their unique electrical and mechanical properties.

How does the fabrication method affect electrode performance?

The fabrication method ensures that the electrodes maintain their integrity and conductivity, which is crucial for effective neural recording.

What is the significance of using Ti₃C₂ MXene?

Ti₃C₂ MXene has high metallic conductivity, making it suitable for applications in neural interfaces.

Can this method be scaled for larger production?

Yes, the method is designed to be reproducible and scalable, allowing for larger production of MXene electrodes.

What applications can benefit from MXene microelectrode arrays?

Applications include in vivo neural recording, brain-computer interfaces, and advanced neuroprosthetics.

Nous décrivons ici une méthode pour fabriquer des tableaux de microélectrode Ti₃C₂ MXene et les utiliser pour l’enregistrement neuronal in vivo.

Ce protocole de synthèse produit de l’encre MXene de haute qualité avec une conductivité métallique qui dépasse les 10 000 Siemens par centimètre. Le principal avantage de cette technique est qu’elle permet le micro-modelage précis des films MXene sans endommager le film ou laisser des résidus nocifs sur les électrodes. Pour préparer une encre carbure de titane, ajoutez lentement deux grammes de carbure en aluminium titane précurseur d’un récipient de réaction de 125 millilitres contenant une solution de gravure sélective et remuez la solution avec une barre magnétique en téflon pendant 24 heures à 35 degrés Celsius à 400 rotations par minute.

À la fin de l’incubation, ajouter 50 millilitres d’eau déionisée à deux tubes centrifugeuses de 175 millilitres, diviser le mélange de réaction de gravure également entre les tubes et remplir à la marque de 150 millilitres. Lavez le matériau par centrifugation répétée, décantant le supernatant acide dans un récipient de déchets dangereux en plastique et ajoutant de l’eau douce aux tubes entre les étapes de centrifugation jusqu’à ce que le pH de la solution supernatale atteigne au-dessus du pH six. Pour que l’intercalation des molécules entre la particule multicouche MXene s’affaiblisse des interactions plane, ajouter deux grammes de chlorure de lithium à 100 millilitres d’eau déionisée et remuer la solution à 200 rotations par minute.

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