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Profilering av H3K4me3 Modifikasjon i planter ved hjelp av spalting under mål og tagmentering

Article DOI: 10.3791/62534-v • 09:48 min • April 22nd, 2022
April 22nd, 2022 •
*1,2, *3, *1, 1,4
1College of Agriculture and Biotechnology, Zhejiang University, 2Central Laboratory, State Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-products, Zhejiang Academy of Agricultural Sciences, 3State Key Laboratory of Crop Genetics and Germplasm Enhancement, Cotton Hybrid R & D Engineering Center (the Ministry of Education), College of Agriculture, Nanjing Agricultural University, 4Hainan Institute of Zhejiang University
* These authors contributed equally

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Cleavage under mål og tagmentering (CUT&Tag) er en effektiv kromatin epigenomisk profileringsstrategi. Denne protokollen presenterer en raffinert CUT&Tag-strategi for profilering av histone modifikasjoner i planter.

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