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Isolering og karakterisering af immuncellerne fra mikrodissekerede musekoroidplexusser

Article DOI: 10.3791/63487-v • 09:55 min • February 3rd, 2022
February 3rd, 2022 •
1, 2, 2, *1, *1
1Brain-Immune Communication Lab, Institut Pasteur, 2Cytometry platform, CB UTechS, Institut Pasteur
* These authors contributed equally

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Denne undersøgelse bruger flowcytometri og to forskellige gating strategier på isolerede perfuserede mus hjerne choroid plexuser; denne protokol identificerer de vigtigste immuncelleundergrupper, der befolker denne hjernestruktur.

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