JOVE JOURNAL

Immunology and Infection

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Journal (Immunology and Infection)

Live/Dead Staining for Quantifying Viable but Not Culturable Cells in Manuka Honey-Treated Wound-Causing Bacteria

Lyric T. Baker¹, Bryn Tennyson¹, Andrea R. Castillo¹

¹Department of Biology,Eastern Washington University

Journal (Immunology and Infection)

Reprograming Model of Human Monocyte-derived Macrophages for In-vitro Assays

Eugenio Antonio Carrera Silva¹, Andrea Emilse Errasti²

¹Instituto de Medicina Experimental (IMEX),Academia Nacional de Medicina-CONICET ²Instituto de Farmacología, Facultad de Medicina,Universidad de Buenos Aires

Journal (Immunology and Infection)

Isolation And Dendritic Cell-Uptake of Small Extracellular Vesicles from Echinococcus granulosus

María Celeste Nicolao^1,2, Maia Chop^2,3, Christian Rodriguez Rodrigues^2,3, Andrea C. Cumino^1,2

¹IIPROSAM, Facultad de Ciencias Exactas y Naturales,Universidad Nacional de Mar del Plata (UNMdP)²Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET)³IQUIBIM, Facultad de Ciencias Exactas y Naturales,Universidad Nacional de Mar del Plata (UNMdP)

Journal (Immunology and Infection)

Preparation of Mycobacterium tuberculosis Culture Filtrate to Understand TB Pathogenesis

Priyadarshini Sanyal^1,2, Vinay Kumar Nandicoori^1,2,3

¹CSIR-Centre for Cellular and Molecular Biology (CSIR-CCMB)²Academy of Scientific and Innovative Research (AcSIR)³National Institute of Immunology

Journal (Immunology and Infection)

Organotypic Tissue Model Systems for Investigating Host-Pathogen Interactions In Vitro

Muhanna Alshehri^1,2, Saeed Alqahtani^1,2,3, Pranitha Murali^2,4,5, Christopher Delaney^1,2, William Johnston^2,4,5, Jason L. Brown^1,2

¹Oral Sciences Research Group, Glasgow Dental School, School of Medicine, College of Medical, Veterinary and Life Sciences,University of Glasgow ²Glasgow Biofilm Research Network ³Department of Restorative Dental Sciences, College of Dentistry,Jouf University ⁴Department of Biological and Biomedical Sciences,Glasgow Caledonian University ⁵Safeguarding Health through Infection Prevention Research Group,Glasgow Caledonian University

Journal (Immunology and Infection)

Droplet-based Cytotoxicity Assay to Assess Chimeric Antigen Receptor T cells at the Single-cell Level

Zelvera Ismetova Usheva¹, David Leander Petersen², Torben Barington¹, Peter Mouritzen², Mike Bogetofte Barnkob¹

¹Centre for Cellular Immunotherapy of Haematological Cancer Odense (CITCO), Department of Clinical Immunology,Odense University Hospital ²Samplix ApS

Journal (Immunology and Infection)

Preparation and Maintenance of Bioexclusion IsoPositive Cage Experiment for Human Fecal Transplantation into Germ-Free Mice

Rachel C. Newsome¹, Chancellor McGriff¹, Raad Z. Gharaibeh¹, Christian Jobin^1,2,3

¹Department of Medicine,University of Florida College of Medicine ²Department of Infectious Diseases and Immunology,University of Florida College of Medicine ³Department of Anatomy and Cell Biology,University of Florida College of Medicine

Journal (Immunology and Infection)

Fluorescence Assays for the Study of Mycobacterium tuberculosis Interaction with the Immune Receptor SLAMF1

Angela María Barbero^1,2, Rodrigo Emanuel Hernández Del Pino^1,2, Verónica Edith García^3,4, Virginia Pasquinelli^1,2

¹Centro de Investigaciones Básicas y Aplicadas (CIBA),Universidad Nacional del Noroeste de la Provincia de Buenos Aires (UNNOBA)²Centro de Investigaciones y Transferencias del Noroeste de la Provincia de Buenos Aires (CIT NOBA),UNNOBA- Universidad Nacional de San Antonio de Areco (UNSAdA)- Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET)³CONICET- Universidad de Buenos Aires, Instituto de Química Biológica de la Facultad de Ciencias Exactas y Naturales (IQUIBICEN)⁴Facultad de Ciencias Exactas y Naturales, Departamento de Química Biológica,Universidad de Buenos Aires

Journal (Immunology and Infection)

Electroporation-Based CRISPR-Cas9-Mediated Gene Knockout in THP-1 Cells and Single-Cell Clone Isolation

Mathieu Pinaud¹, Alessia Zamborlini¹

¹INSERM, CEA, Center for Immunology of Viral, Auto-immune, Hematological and Bacterial diseases (IMVA-HB/IDMIT),Université Paris-Saclay

Journal (Immunology and Infection)

Prion Safety Laboratory Swipe Test

Sara M. Simmons¹, Qi Yuan¹, Jason C. Bartz¹

¹Department of Medical Microbiology and Immunology, School of Medicine,Creighton University

Journal (Immunology and Infection)

Advances in Human Induced Pluripotent Stem Cell-Derived Chimeric Antigen Receptor-Expressing Natural Killer Cells

Jaya Lakshmi Thangaraj¹, Dan S. Kaufman^1,2

¹Department of Medicine, Division of Regenerative Medicine,University of California, San Diego ²Sanford Stem Cell Institute,University of California, San Diego

Journal (Immunology and Infection)

Prediction of Red Blood Cell Antibody Significance Using the Monocyte-Macrophage Assay

Lindsay K. Hillis^1,2, Selena Cen², Clemence Salou³, Yeniley Ruiz Noa², Donald R. Branch^1,2,4

¹Department of Laboratory Medicine and Pathobiology,University of Toronto ²Centre for Innovation, Canadian Blood Services,Keenan Research Centre ³Polytech Clermont ⁴Department of Medicine,University of Toronto

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