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Introduction to the Spectrophotometer

JoVE 5038

The spectrophotometer is a routinely used instrument in scientific research. Spectrophotometry is the quantitative measurement of how much a chemical substance absorbs light by passing a beam of light through the sample using a spectrophotometer.

In this video, basic concepts in spectrophotometry, including transmittance, absorbance and the Beer-Lambert Law …

 General Laboratory Techniques

Measuring Mass in the Laboratory

JoVE 5037

The analytical balance is the common piece of equipment in the scientific laboratory. These highly precise instruments can measure down to ten thousandths or even hundred thousandths of a gram. The triple beam balance is a type of scale that uses counterweights on a three rider beams to counterbalance the sample in the pan. On the other hand, modern analytical balances…

 General Laboratory Techniques

An Introduction to Working in the Hood

JoVE 5036

Hoods are a common class of instrument, which act as a protective enclosure for various types of laboratory experiments. They serve to shield experimental samples from the environment, as well as to protect the researcher from hazardous or infectious samples. “The Hood” is a term that can include laminar flow benches, fume hoods, as well as tissue culture…

 General Laboratory Techniques

Introduction to the Bunsen Burner

JoVE 5035

The Bunsen burner, named after and co-designed by Robert Bunsen in 1854, is a common laboratory instrument that produces a hot, sootless, non-luminous flame. The Bunsen Burner allows for precise regulation of the mixing of gas and oxygen in its central barrel before combustion, which ignites the flame. By manipulating the Bunsen burner, both the size and temperature of the …

 General Laboratory Techniques

Introduction to Serological Pipettes and Pipettors

JoVE 5034

The serological pipette is frequently used in the laboratory for transferring milliliter volumes of liquid, from less than 1 ml to up to 50 ml. The pipettes can be sterile, plastic, and disposable or sterilizable, glass and reusable. Both kinds of pipettes use a pipet-aid, for the aspiration and dispensation of liquids. Different sizes of pipettes can be used with the same …

 General Laboratory Techniques

An Introduction to the Micropipettor

JoVE 5033

The micropipettor is a common laboratory instrument used for transferring microvolumes of liquid solutions. Micropipettors come in a range of sizes for the accurate movement of volumes between 0.5 and 5000 μl and each instrument requires one of three different sized disposable tips. Micropipettors work by displacing air from the pipette shaft, allowing the liquid to …

 General Laboratory Techniques

Making Solutions in the Laboratory

JoVE 5030

The ability to successfully make solutions is a basic laboratory skill performed in virtually all biological and chemical experiments. A solution is a homogenous mixture of solute dissolved in bulk liquid known as the solvent. Solutions can be described by their solute concentration, a measure of how much solute is present per unit of solution.

In this…

 General Laboratory Techniques

Understanding Concentration and Measuring Volumes

JoVE 5026

Solutions are utilized to some degree in almost all biological research applications. Therefore understanding how to measure and manipulate them is imperative to any experiment. In this video, concepts in preparing solutions are introduced.

Solutions consist of a solute dissolved in solvent to yield a homogeneous mixture of molecular substances. …

 General Laboratory Techniques

Introduction to the Microplate Reader

JoVE 5024

The microplate reader is a multimodal instrument that allows for a variety of experiments to be performed and measured simultaneously. Microplate readers can make absorbance, fluorescence and luminescence measurements. Multiwell plates are integral to the microplate reader and allow for many experiments to be performed at once. Regardless of the assay type, experiments…

 General Laboratory Techniques

An Introduction to the Centrifuge

JoVE 5019

The centrifuge is an instrument used in nearly every research lab across the globe. Centrifugation is the process by which a centrifuge is used to separate components of a complex mixture. By spinning laboratory samples at very high speeds, the components of a given mixture are subjected to centrifugal force, which causes more dense particles to migrate away from the axis of rotation and…

 General Laboratory Techniques
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