Laboratoire National d'Appui au Developpement Agricole Bingerville 1 article published in JoVE Immunology and Infection Field Postmortem Rabies Rapid Immunochromatographic Diagnostic Test for Resource-Limited Settings with Further Molecular Applications Stephanie Mauti1, Monique Léchenne2, Service Naïssengar3, Abdallah Traoré4, Vessaly Kallo5,6, Casimir Kouakou7, Emmanuel Couacy-Hymann7, Morgane Gourlaouen8, Céline Mbilo9,10, Pati Patient Pyana11, Enos Madaye3, Ibrahima Dicko4, Pascal Cozette1, Paola De Benedictis8, Hervé Bourhy1, Jakob Zinsstag9,10, Laurent Dacheux1 1Unit Lyssavirus Epidemiology and Neuropathology, National Reference Center for Rabies and WHO Collaborating Center for Reference and Research on Rabies, Institut Pasteur, 2Environment and Sustainability Institute, University of Exeter, Penryn Campus, 3Institut de Recherche en Elevage pour le Développement, 4Laboratoire Central Vétérinaire, 5 We present a complete protocol for postmortem diagnosis of animal rabies under field conditions using a rapid immunochromatographic diagnostic test (RIDT), from brain biopsy sampling to final interpretation. We also describe further applications using the device for molecular analysis and viral genotyping.
