United Arab Emirates University 2 articles published in JoVE Biology Lentiviral Mediated Delivery of shRNAs to hESCs and NPCs Using Low-cost Cationic Polymer Polyethylenimine (PEI) Muhammad Abid Sheikh1, Suraiya Anjum Ansari1,2 1Department of Biochemistry and Molecular Biology, College of Medicine and Health Sciences, United Arab Emirates University, 2Zayed Center for Health Sciences, United Arab Emirates University Using the low-cost cationic polymer polyethylenimine (PEI), we produced lentiviral particles for stable expression of shRNAs in H9 human embryonic stem cells (hESCs) and transiently transduced H9-derived neural progenitor cells (NPCs) at high efficiency. Biology High-Throughput Metabolic Profiling for Model Refinements of Microalgae Amnah Alzahmi1,2, Sarah Daakour1, Diana Charles El Assal3, Bushra S. Dohai3, Amphun Chaiboonchoe3, Weiqi Fu3,4, David R. Nelson1, Alexandra Mystikou1, Kourosh Salehi-Ashtiani1,3 1Center for Genomics and Systems Biology (CGSB), New York University Abu Dhabi Research Institute, 2Department of Biology, United Arab Emirates University, 3Laboratory of Algal, Systems, and Synthetic Biology (LASSB), Division of Science and Math, New York University Abu Dhabi, 4Department of Marine Science, Ocean College, Zhejiang University This protocol demonstrates the use of a phenotype microarray (PM) technology platform to define metabolic requirements of Chlamydomonas reinhardtii, a green microalga, and refine an existing metabolic network model.
