University of Science and Technology Chittagong (USTC) 1 article published in JoVE Immunology and Infection Quantifying Myeloperoxidase-DNA and Neutrophil Elastase-DNA Complexes from Neutrophil Extracellular Traps by Using a Modified Sandwich ELISA Md. Monirul Islam1, Umme Salma2, Takayuki Irahara2, Eizo Watanabe2, Naoshi Takeyama2 1Department of Biochemistry and Biotechnology, University of Science and Technology Chittagong (USTC), 2Department of Emergency and Critical Care Medicine, Aichi Medical University We present a protocol for a modified sandwich enzyme-linked immunosorbent assay technique to quantitatively measure two components of neutrophil extracellular trap remnants, myeloperoxidase conjugated-DNA and neutrophil elastase conjugated-DNA complexes,derived from activated neutrophils.
