Harbin Medical University View Institution's Website 4 articles published in JoVE Bioengineering Propagation of Dental and Respiratory Cells and Organs in Microgravity Mirali Pandya1, Wei Ma1,2, Huling Lyu1,3, Xianghong Luan1, Thomas G. H. Diekwisch1 1Center for Craniofacial Research and Diagnosis, Texas A&M College of Dentistry, 2Department of Stomatology, The Fourth Affiliated Hospital, Harbin Medical University, 3Key Laboratory of Oral Medicine, Guangzhou Insititute of Oral Disease, Stomatology Hospital of Guangzhou Medical University This protocol presents a method for the culture and 3D growth of ameloblast-like cells in microgravity to maintain their elongated and polarized shape as well as enamel-specific protein expression. Culture conditions for the culture of periodontal engineering constructs and lung organs in microgravity are also described. Immunology and Infection Blocking Lymph Flow by Suturing Afferent Lymphatic Vessels in Mice Yujia Lin1,2, Jingna Xue2, Shan Liao2 1Department of General Surgery, The Second Affiliated Hospital of Harbin Medical University, Harbin Medical University, 2Inflammation Research Network, Department of Microbiology, Immunology and Infectious Diseases, Snyder Institute for Chronic Diseases, Cumming School of Medicine, University of Calgary A protocol to block lymph flow by surgical suturing of afferent lymphatic vessels is presented. Biology Detecting Establishment of Shared Blood Supply in Parabiotic Mice by Caudal Vein Glucose Injection Xin Liu1, Xue Bai1, Mingqi Li1, Huimin Li1, Yong Zhang1,2, Baofeng Yang1,3 1Department of Pharmacology, The State-Province Key Laboratories of Biomedicine-Pharmaceutics of China, Key Laboratory of Cardiovascular Research, Ministry of Education, College of Pharmacy, Harbin Medical University, 2Institute of Metabolic Disease, Heilongjiang Academy of Medical Science, 3Department of Pharmacology and Therapeutics, Melbourne School of Biomedical Sciences, Faculty of Medicine, Dentistry and Health Sciences University of Melbourne Here we describe a new method of detecting successful establishment of shared blood circulation of two parabionts through a caudal vein injection of glucose, which causes minimal damage and is not fatal to the parabionts. Neuroscience An Optic Nerve Crush Injury Murine Model to Study Retinal Ganglion Cell Survival Zhongshu Tang1, Shuihua Zhang1,2, Chunsik Lee1, Anil Kumar1, Pachiappan Arjunan1, Yang Li1, Fan Zhang1, Xuri Li1 1National Eye Institute, NIH, 2Ophthalmology Department, The Second Hospital of Harbin Medical University This protocol shows how to retrogradely label retinal ganglion cells, and how to subsequently make an optic nerve crush injury in order to analyze retinal ganglion cell survival and apoptosis. It is an experimental disease model for different types of optic neuropathy, including glaucoma.
