Second Military Medical University 4 articles published in JoVE Immunology and Infection Clarifying and Imaging Candida albicans Biofilms Frederick Lanni1, Katherine Lagree1, Manning Y. Huang1, Lan Yan2, Carol A. Woolford1, Aaron P. Mitchell1 1Department of Biological Sciences, Carnegie Mellon University, 2Department of Pharmacology, Second Military Medical University To view and quantify the internal features of Candida albicans biofilms, we prepare fixed intact specimens that are clarified by refractive index matching. Then, optical sectioning microscopy can be used to obtain three-dimensional image data though the full thickness of the biofilm. Behavior Assessing the Autonomic and Behavioral Effects of Passive Motion in Rats using Elevator Vertical Motion and Ferris-Wheel Rotation Francis A. M. Manno1,2, Leilei Pan3, Yuqi Mao3, Yang Su3, Sinai H. C. Manno2,4,5, Shuk Han Cheng4,5,6, Condon Lau2, Yiling Cai3 1School of Biomedical Engineering, Faculty of Engineering, University of Sydney, 2Department of Physics, City University of Hong Kong, 3Department of Nautical Injury Prevention, Faculty of Navy Medicine, Second Military Medical University, 4State Key Laboratory of Marine Pollution (SKLMP), City University of Hong Kong, 5Department of Biomedical Sciences, College of Veterinary Medicine and Life Sciences, City University of Hong Kong, 6Department of Materials Science and Engineering, City University of Hong Kong Protocols are presented to assess the autonomic and behavioral effects of passive motion in rodents using elevator vertical motion and Ferris-wheel rotation. Developmental Biology Dissection and Coronal Slice Preparation of Developing Mouse Pituitary Gland Dongmei Cao1, Xianhua Ma1, Weiping J. Zhang1, Zhifang Xie1,2 1Department of Pathophysiology, Second Military Medical University, 2Department of Cell Biology, Second Military Medical University We present a protocol to dissect pituitary glands and prepare pituitary coronal sections from developing mice. Medicine The Inverted Heart Model for Interstitial Transudate Collection from the Isolated Rat Heart Kezhe Tan*1,2, Zhaoping Ding*1,2, Bodo Steckel2, Sonja Hartwig3, Stefan Lehr3, Xiaoming Deng1, Jürgen Schrader2 1Department of Anesthesiology, Changhai Hospital, Second Military Medical University, 2Department of Molecular Cardiology, Heinrich Heine University, 3Department of Clinical Biochemistry and Pathobiochemistry, German Diabetes Center, Heinrich Heine University This protocol describes a method to collect cardiac interstitial fluid from the isolated, perfused rat heart. To physically separate interstitial transudate from coronary venous effluent perfusate, the Langendorff perfused heart is inverted, and the transudate (interstitial fluid) formed on the cardiac surface is collected using a soft latex cap.
