Jiangsu Collaborative Innovation Center of Regional Modern Agriculture and Environmental Protection 1 article published in JoVE Bioengineering Visual Detection of Multiple Nucleic Acids in a Capillary Array Jianwei Chen*1,2,3, Ning Shao*1,2,3, Jiaying Hu*4, Rong Li4, Yuanshou Zhu1,2,3, Dabing Zhang4,5, Shujuan Guo1,2,3, Junhou Hui6, Peng Liu6, Litao Yang4, Sheng-Ce Tao1,2,3 1Key Laboratory of Systems Biomedicine (Ministry of Education), Shanghai Center for Systems Biomedicine, Shanghai Jiao Tong University, 2State Key Laboratory of Oncogenes and Related Genes, 3School of Biomedical Engineering, Shanghai Jiao Tong University, 4Collaborative Innovation Center for Biosafety of GMOs, National Center for the Molecular Characterization of Genetically Modified Organisms, School of Life Sciences and Biotechnology, Shanghai Jiao Tong University, 5Key Laboratory of Crop Marker-Assisted Breeding of Huaian Municipality, Jiangsu Collaborative Innovation Center of Regional Modern Agriculture and Environmental Protection, 6Department of Biomedical Engineering, Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, School of Medicine, Tsinghua University This protocol describes the fabrication of a small, ready-to-use cassette that can be applied for visual detection of multiple nucleic acids in a single, test that is easy to operate. In this approach, a capillary array was used for multiplex and highly efficient detection of GMO targets.
