Ministry of Agriculture 4 articles published in JoVE Biochemistry Extracting Venom from the Parasitoid Wasp Trichogramma dendrolimi Using an Artificial Host Hai-yan Wang*1,2, Ze-qi Yu*1,2, Xin-yue Ren1,2, Yuan-xi Li1,2, Zhi-chao Yan1,2,3 1Department of Entomology, Nanjing Agricultural University, 2Key Laboratory of Integrated Pest Management of Crop of East China, Ministry of Agriculture, 3Sanya Institute of Nanjing Agricultural University Here, we present a protocol for extracting venom from Trichogramma dendrolimi using an artificial host created with polyethylene film and amino acid solution. Genetics An Effective Inoculation Method for Phytophthora capsici on Black Pepper Plants YueFeng Su*1,2, Rui Fan*1,3, Li-Song Hu1,4, Bao-Duo Wu1,3, Chao-Yun Hao1 1Spice and Beverage Research Institute, Chinese Academy of Tropical Agricultural Science (CATAS), 2Hainan University, 3Key Laboratory of Genetic Resources Utilization of Spice and Beverage Crops, Ministry of Agriculture, 4Hainan Provincial Key Laboratory of Genetic Improvement and Quality Regulation for Tropical Spice and Beverage Crops Pinpricking the basal head of the black pepper plant is a brief and time-saving method to damage it. Here, we provided detailed steps with a video for infecting black pepper plants. Immunology and Infection Intracranial Subarachnoidal Route of Infection for Investigating Roles of Streptococcus suis Biofilms in Meningitis in a Mouse Infection Model Shouming Zhang1,2,3, Xueping Gao1,2,3, Genhui Xiao1,2,3,4, Chengping Lu1,2,3, Huochun Yao1,2,3, Hongjie Fan1,2,3,5, Zongfu Wu1,2,3 1College of Veterinary Medicine, Nanjing Agricultural University, 2Key Lab of Animal Bacteriology, Ministry of Agriculture, 3OIE Reference Lab for Swine Streptococcosis, 4School of Medicine, Tsinghua University, 5Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses Here, we describe the intracranial subarachnoidal route of infection in mice to study roles of biofilms in Streptococcus suis meningitis. This infection model is also suitable for studying the pathogenesis of other bacterial meningitis and the efficacy of new drugs against bacterial meningitis. Immunology and Infection Isolation and Characterization of Extracellular Vesicles from Adult Schistosoma japonicum Juntao Liu*1, Lihui Zhu*1, Lihui Wang1,2, Yongjun Chen1, Bikash Ranjan Giri1, Jianjun Li2, Guofeng Cheng1 1Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Key Laboratory of Animal Parasitology, Ministry of Agriculture, 2Tianjin Agricultural University Here, we present a protocol to describe extracellular vesicles (EVs) isolation in in vitro cultured medium from adult Schistosoma japonicum.
