Chinese Center for Disease Control and Prevention 2 articles published in JoVE Immunology and Infection Stability and Structure of Bat Major Histocompatibility Complex Class I with Heterologous β2-Microglobulin Di Zhang*1,2, Kefang Liu*3,4, Dan Lu*2,5, Pengyan Wang1,2, Qingxu Zhang1,2, Peipei Liu2, Yingze Zhao2, Yan Chai4, Jianxin Lyu1, Jianxun Qi4, William J. Liu1,2 1School of Laboratory Medicine and Life Sciences, Wenzhou Medical University, 2NHC Key Laboratory of Biosafety, National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, 3Faculty of Health Sciences, University of Macau, 4CAS Key Laboratory of Pathogenic Microbiology and Immunology, Institute of Microbiology, Chinese Academy of Sciences, 5Savaid Medical School, University of Chinese Academy of Sciences The protocol describes experimental methods to obtain stable major histocompatibility complex (MHC) class I through potential β2-microglobulin (β2m) substitutions from different species. The structural comparison of MHC I stabilized by homologous and heterologous β2m were investigated. Immunology and Infection Evaluation of Zika Virus-specific T-cell Responses in Immunoprivileged Organs of Infected Ifnar1-/- Mice Yongli Zhang*1,2, Hangjie Zhang*2, Wenqiang Ma*3, Kefang Liu1,2, Min Zhao4, Yingze Zhao2, Xuancheng Lu5, Fuping Zhang6, Xiangdong Li3, George F. Gao1,2,4,6, William J. Liu1,2 1School of Laboratory Medicine and Life Sciences, Wenzhou Medical University, 2NHC Key Laboratory of Medical Virology and Viral Diseases, National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, 3State Key Laboratory of Agrobiotechnology, College of Biological Sciences, China Agricultural University, 4Research Network of Immunity and Health (RNIH), Beijing Institutes of Life Science, Chinese Academy of Sciences, 5Laboratory Animal Center, Chinese Center for Disease Control and Prevention, 6CAS Key Laboratory of Pathogenic Microbiology and Immunology, Institute of Microbiology, Chinese Academy of Sciences A protocol to evaluate antigen-specific T-cell responses in the immunoprivileged organs of the Ifnar1-/- murine model for the Zika virus (ZIKV) infection is described. This method is pivotal for investigating the cellular mechanisms of the protection and immunopathogenesis of ZIKV vaccines and is also valuable for their efficacy evaluation.
