First Affiliated Hospital of Jinan University 5 articles published in JoVE Medicine Establishment and Evaluation of a Porcine Vein Graft Disease Model Xiaohui Li*1, Jinlin Hu*1, Weijiang Tan*2, Zhaoming Lin*1, Caiyi Zhu2, Chengfeng Huang1, Jiawen Huang3, Yintian Liu1, Qiuying Liao1, Hua Lu1, Xiaoshen Zhang1 1Department of Cardiovascular Surgery, the First Affiliated Hospital of Jinan University, 2Guangdong Provincial Key Laboratory of Laboratory Animals, Guangdong Laboratory Animals Monitoring Institute, 3The First Affiliated Hospital of Jinan University In this protocol, novel pig vein bypass grafting was performed through a small incision in the left chest wall without cardiopulmonary bypass. A postoperative pathology study was done, which showed intimal thickening. Neuroscience Dissecting Cell-Autonomous Function of Fragile X Mental Retardation Protein in an Auditory Circuit by In Ovo Electroporation Qiwei Fan*1, Xiaotan Zhang*2, Yuan Wang3, Xiaoyu Wang1 1Division of Histology & Embryology, Key Laboratory for Regenerative Medicine of the Ministry of Education, College of Medicine, Jinan University, 2Department of Clinical Pathology, First Affiliated Hospital of Jinan University, 3Program in Neuroscience, Department of Biomedical Sciences, College of Medicine, Florida State University Using in ovo electroporation, we devised a method to selectively transfect the auditory inner ear and cochlear nucleus in chicken embryos to achieve a cell-group-specific knockdown of fragile X mental retardation protein during discrete periods of circuit assembly. Medicine A Surgical Model of Heart Failure with Preserved Ejection Fraction in Tibetan Minipigs Xiaohui Li*1,2, Weijiang Tan*1,3, Xiang Li*1, Shuang Zheng*1, Xiaoshen Zhang2, Honghua Chen1, Zhanhong Pan1, Caiyi Zhu1, Feng Hua Yang1 1Guangdong Laboratory Animals Monitoring Institute, 2Department of Cardiovascular Surgery, the First Affiliated Hospital, Jinan University, 3College of Veterinary Medicine, South China Agricultural University The present protocol describes a step-by-step procedure to establish a minipig model of heart failure with preserved ejection fraction using descending aortic constriction. The methods for evaluating cardiac morphology, histology, and function of this disease model are also presented. Bioengineering Construction of a Multilayered Mesenchymal Stem Cell Sheet with a 3D Dynamic Culture System Yingwei Wang*1,2, Cheng Lu*1,2, Chengzhi He3, Baoxin Chen1,2, Youling Zheng1,2, Junming Zheng1,2, Jianhua Zhang4, Zheng Wu1,2 1Key Laboratory for Regenerative Medicine, Ministry of Education, Jinan University, 2Department of Developmental and Regenerative Biology, Jinan University, 3Nansha College Preparatory Academy, 4Department of Cardiology, First Affiliated Hospital of Jinan University This article provides an efficient and feasible method for constructing multilayered stem cell sheets with favorable stem cell property. Immunology and Infection A Silver Nanoparticle Method for Ameliorating Biliary Atresia Syndrome in Mice Ming Fu1, Zefeng Lin2, Huiting Lin2, Yanlu Tong2, Hezhen Wang2, Hongjiao Chen2, Yan Chen2, Ruizhong Zhang2 1First Affiliated Hospital of Jinan University, 2Department of Pediatric Surgery, Guangzhou Women & Children's Medical Center, Guangzhou Medical University This article describes in detail a method based on silver nanoparticles for ameliorating biliary atresia syndrome in an experimental biliary atresia mouse model. A solid understanding of the reagent preparation process and the neonatal mouse injection technique will help familiarize researchers with the method used in neonatal mouse model studies.
