Zhejiang Academy of Agricultural Sciences 3 articles published in JoVE Biology Profiling of H3K4me3 Modification in Plants using Cleavage under Targets and Tagmentation Xiaoyuan Tao*1,2, Mengtao Gao*3, Siyuan Wang*1, Xueying Guan1,4 1College of Agriculture and Biotechnology, Zhejiang University, 2Central Laboratory, State Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-products, Zhejiang Academy of Agricultural Sciences, 3State Key Laboratory of Crop Genetics and Germplasm Enhancement, Cotton Hybrid R & D Engineering Center (the Ministry of Education), College of Agriculture, Nanjing Agricultural University, 4Hainan Institute of Zhejiang University Cleavage under targets and tagmentation (CUT&Tag) is an efficient chromatin epigenomic profiling strategy. This protocol presents a refined CUT&Tag strategy for the profiling of histone modifications in plants. Biology Screening of Tobacco Genotypes for Phytophthora nicotianae Resistance Yutong Liu1, Mingming Sun1, Zipeng Jiang1, Xinfang Wang2, Bingguang Xiao3, Aiguo Yang1, He Meng1, Lirui Cheng1 1Key Laboratory of Tobacco Genetic Improvement and Biotechnology, Tobacco Research Institute, Chinese Academy of Agricultural Sciences, 2Institute of Rural Development, Zhejiang Academy of Agricultural Sciences, 3Yunnan Academy of Tobacco Agricultural Sciences Here, a protocol is presented for the efficient and accurate screening of tobacco genotypes for Phytophthora nicotianae resistance in seedlings. This is a practical approach for precision breeding, as well as molecular mechanism research. Environment Generating Homo- and Heterografts Between Watermelon and Bottle Gourd for the Study of Cold-responsive MicroRNAs Lingping Wang*1, Xinyi Wu*1, Guojing Li1,2, Xiaohua Wu1, Dehui Qin3, Ye Tao4, Pei Xu1,2 1Institute of Vegetables, Zhejiang Academy of Agricultural Sciences, 2State Key Lab Breeding Base for Sustainable Control of Plant Pest and Disease, Zhejiang Academy of Agricultural Sciences, 3Institute of Horticulture, Zhejiang Academy of Agricultural Sciences, 4Shanghai Biozeron Biotechnology Co., Ltd Here we present a detailed protocol for efficiently making homo- and heterografts between watermelon and bottle gourd, in addition to methods of tissue sampling, data generation, and data analysis, for the investigation of cold-responsive microRNAs.
