Guangdong Academy of Medical Sciences, Guangdong General Hospital 3 articles published in JoVE Medicine Preparation, Procedures and Evaluation of Platelet-Rich Plasma Injection in the Treatment of Knee Osteoarthritis Ziming Chen*1,2, Zhantao Deng*1, Yuanchen Ma1, Junxing Liao1, Qingtian Li1, Mengyuan Li1, Hua Liu1, Ganghong Chen1, Chaoming Zeng1, Qiujian Zheng1 1Department of Orthopedics, Guangdong General Hospital (Guangdong Academy of Medical Sciences), 2Shantou University Medical College Knee osteoarthritis is frequently seen in the orthopedic department. We introduce in detail the entire knee osteoarthritis treatment process with platelet-rich plasma injection, including preparation, procedures, and evaluation. Bioengineering Nondestructive Monitoring of Degradable Scaffold-Based Tissue-Engineered Blood Vessel Development Using Optical Coherence Tomography Wanwen Chen1, Shangmin Liu2, Junqing Yang1, Yueheng Wu2, Wentao Ma2, Zhanyi Lin3 1Department of Cardiology, Guangdong Academy of Medical Sciences, Guangdong General Hospital, 2Medical Research Center, Guangdong Academy of Medical Sciences, Guangdong General Hospital, 3Institute of Geriatric medicine, Guangdong Academy of Medical Sciences, Guangdong General Hospital A step by step protocol for nondestructive and long-period monitoring the process of vascular remodeling and scaffold degradation in real-time culture of biodegradable polymeric scaffold-based tissue-engineered blood vessels with pulsatile stimulation using optical coherence tomography is described here. Developmental Biology Rapid Acquisition of 3D Images Using High-resolution Episcopic Microscopy Haochuan Zhang*1,2,3, JunGang Huang*2,3,4, Xin Liu2,3, Ping Zhu4, Zhongrong Li1, Xue Li2,3 1Department of Pediatric Surgery, The Second Affiliated Hospital & Yuying Children's Hospital, Wenzhou Medical University, 2 We describe a detailed protocol using high-resolution episcopic microscopy to acquire three-dimensional (3D) images of mouse embryos. This improved protocol utilizes a modified tissue preparation method to enhance penetration of the fluorescent dye, thereby permitting morphometric analysis of both small and large-sized specimens.
