Renmin Hospital of Wuhan University 3 articles published in JoVE Neuroscience Methanol-Based Whole-Mount Preparation for the Investigation of Retinal Ganglion Cells Ningzhi Zhang1, Zhiyi Wang1, Pei Lin1, Yiqiao Xing1, Ning Yang1 1Department of Ophthalmology, Renmin Hospital of Wuhan University Methanol can be used as an auxiliary fixed medium for retinal whole-mount preparations and long-term storage, which is useful for the investigation of retinal ganglion cells. Cancer Research Three Differential Expression Analysis Methods for RNA Sequencing: limma, EdgeR, DESeq2 Shiyi Liu*1, Zitao Wang*1, Ronghui Zhu1, Feiyan Wang2, Yanxiang Cheng1, Yeqiang Liu2 1Department of Obstetrics and Gynecology, Renmin Hospital of Wuhan University, 2Department of Pathology, Shanghai Skin Disease Hospital, Tongji University School of Medicine A detailed protocol of differential expression analysis methods for RNA sequencing was provided: limma, EdgeR, DESeq2. Cancer Research Digital Analysis of Immunostaining of ZW10 Interacting Protein in Human Lung Tissues Yuan Wen1, Xie Song-ping2, Liu Pan3, Liu Xiao-yan1, Pan Shan4, Yin Qian4, Sun Meng5, Huang Xiao-xing6, Xiao Rui-jing4, Xiong Jie4, Zhang Qiu-ping4, Shao Liang1 1Department of Hematology, Zhongnan Hospital of Wuhan University, 2Department of Thoracic Surgery, Renmin Hospital of Wuhan University, 3Department of Human Anatomy, Histology and Embryology, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences, School of Basic Medicine, Peking Union Medical College, 4Department of Immunology, School of Basic Medical Sciences, Wuhan University, 5Department of Gastroenterology, Central Hospital of Wuhan, 6Department of Transfusion, Zhongnan Hospital of Wuhan University ZW10 interacting protein (ZWINT) participates in the mitotic spindle checkpoint and the pathogenesis of carcinoma. Here, we introduce a methodology of the immunostaining of ZWINT in human lung cancer tissues, followed by the digital scanning of whole slides and image analysis. This methodology can provide high-quality digital images and reliable results.
