Central Hospital of Wuhan 1 article published in JoVE Cancer Research Digital Analysis of Immunostaining of ZW10 Interacting Protein in Human Lung Tissues Yuan Wen1, Xie Song-ping2, Liu Pan3, Liu Xiao-yan1, Pan Shan4, Yin Qian4, Sun Meng5, Huang Xiao-xing6, Xiao Rui-jing4, Xiong Jie4, Zhang Qiu-ping4, Shao Liang1 1Department of Hematology, Zhongnan Hospital of Wuhan University, 2Department of Thoracic Surgery, Renmin Hospital of Wuhan University, 3Department of Human Anatomy, Histology and Embryology, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences, School of Basic Medicine, Peking Union Medical College, 4Department of Immunology, School of Basic Medical Sciences, Wuhan University, 5Department of Gastroenterology, Central Hospital of Wuhan, 6Department of Transfusion, Zhongnan Hospital of Wuhan University ZW10 interacting protein (ZWINT) participates in the mitotic spindle checkpoint and the pathogenesis of carcinoma. Here, we introduce a methodology of the immunostaining of ZWINT in human lung cancer tissues, followed by the digital scanning of whole slides and image analysis. This methodology can provide high-quality digital images and reliable results.
